Cat. No.
0107-19

Size
0.1 mg

Price (USD)
$165.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Source	Normal mouse serum
Format/Conjugate	APC/CY7 (Allophycocyanin/Cyanine 7)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light; Do not freeze Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include - Flow Cytometry ^7-10 ELISA ^1-6 FLISA Other referenced applications for relevant formats include - Immunohistochemistry-Paraffin Sections ¹¹ Immunocytochemistry ¹² Western Blot ^13,14 Immunoprecipitation ^15,16 Chromatin Immunoprecipitation ^17,18 Blocking ^19,20 In vitro Control ^22-25 In vivo Control ^2,9,21
Recommended Dilutions	Please refer to product specific Technical Bulletin

FLISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG-APC (SB Cat. No. 0107-11) was captured and fluorescence intensity quantified.

FLISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG-APC (SB Cat. No. 0107-11) was captured and fluorescence intensity quantified.

References

1.	Kuklin N, Daheshia M, Karem K, Manickan E, Rouse BT. Induction of mucosal immunity against herpes simplex virus by plasmid DNA immunization. J Virol. 1997;71:3138-45. (ELISA)
2.	Cupples AL. Addition of an anti-DNA antibody enhances plasmid DNA vaccination against herpes simplex virus [thesis]. Knoxville (USA): University of Tennessee; 2004. (ELISA, in vivo control)
3.	Genestier L, Taillardet M, Mondiere P, Gheit H, Bella C, Defrance T. TLR agonists selectively promote terminal plasma cell differentiation of B cell subsets specialized in thymus-independent responses. J Immunol. 2007;178:7779-86. (ELISA)
4.	Wang Y, Diamond B. B cell receptor revision diminishes the autoreactive B cell response after antigen activation in mice. J Clin Invest. 2008;118:2896-907. (ELISA)
5.	Szymczak WA, Davis MJ, Lundy SK, Dufaud C, Olszewski M, Pirofski L. X-linked immunodeficient mice exhibit enhanced susceptibility to Cryptococcus neoformans infection. Mbio. 2013;4(4): e00265-13. (ELISA)
6.	Mann JF, McKay PF, Fiserova A, Klein K, Cope A, Rogers P, et al. Enhanced immunogenicity of an HIV-1 DNA vaccine delivered with electroporation via combined intramuscular and intradermal routes. J Virol. 2014;88:6959-69. (ELISA)
7.	Nataf S, Davoust N, Ames RS, Barnum SR. Human T cells express the C5a receptor and are chemoattracted to C5a. J Immunol. 1999;162:4018-23. (FC)
8.	Butler JE, Moore MB, Presnell SR, Chan H, Chalupny NJ, Lutz CT. Proteasome regulation of ULBP1 transcription. J Immunol. 2009;182:6600-9. (FC)
9.	O'Neill AF, Dearling JL, Wang Y, Tupper T, Sun Y, Aster JC, et al. Targeted imaging of Ewing sarcoma in preclinical models using a ⁶⁴Cu-labeled anti-CD99 antibody. Clin Cancer Res. 2014;20:678-87. (FC, in vivo control)
10.	Silvescu CI, Sackstein R. G-CSF induces membrane expression of a myeloperoxidase glycovariant that operates as an E-selectin ligand on human myeloid cells. Proc Natl Acad Sci USA. 2014;111:10696-701. (FC)
11.	Howell MD, Kim BE, Gao P, Grant AV, Boguniewicz M, DeBenedetto A, et al. Cytokine modulation of atopic dermatitis filaggrin skin expression. J Allergy Clin Immunol. 2007;120:150-5. (IHC-PS)
12.	Hauser SP, Waldron JA, Upuda KB, Lipschitz DA. Morphological characterization of stromal cell types in hematopoietically active long-term murine bone marrow cultures. J Histochem Cytochem. 1995;43:371-9. (ICC)
13.	Lintomen L, Elsas MI, Maximiano ES, de Paula Neto HA, Joseph D, Vargaftig BB, et al. Allergenic sensitization prevents upregulation of haemopoiesis by cyclo-oxygenase inhibitors in mice. Br J Pharmacol. 2002;135:1315-23. (WB)
14.	Xu J, Cui G, Esmailian F, Plunkett M, Marelli D, Ardehali A, et al. Atrial extracellular matrix remodeling and the maintenance of atrial fibrillation. Circulation. 2004;109:363-8. (WB)
15.	Inagaki T, Iwaski S, Matsumura Y, Kawamura T, Tanaka T, Abe Y, et al. The FBXL10/KDM2B scaffolding protein associates with novel polycomb repressive complex-1 to regulate adipogenesis. J Biol Chem. 2015;290:4163-77. (IP)
16.	Li G, Nguyen CC, Ryckman BJ, Britt WJ, Kamil JP. A viral regulator of glycoprotein complexes contributes to human cytomegalovirus cell tropism. Proc Natl Acad Sci USA. 2015;112:4471-6. (IP)
17.	Breslin MB, Wang H, Pierce A, Aucoin R, Lan MS. Neurogenin 3 recruits CBP co-activator to facilitate histone H3/H4 acetylation in the target gene INSM1. FEBS Lett. 2007;581:949-54. (ChIP)
18.	Tao Y, Sharif N, Zeng B, Cai Y, Guo Y. Lateral ventricle injection of orexin-A ameliorates central precocious puberty in rat via inhibiting the expression of MEG3. Int J Clin Exp Pathol. 2015;8:12564-70. (ChIP)
19.	Bühring H, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007;1106:262-71. (Block)
20.	Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, et al. A pathobiological role of the insulin receptor in chronic lymphocytic leukemia. Clin Cancer Res. 2011;17:2679-92. (Block)
21.	Arumugam T, Deng D, Bover L, Wang H, Logsdon CD, Ramachandran V. New blocking antibodies against novel AGR2-C4.4A pathway reduce growth and metastasis of pancreatic tumors and increase survival in mice. Mol Cancer Ther. 2015;14:941-51. (In vivo control)
22.	Nakajima H, Samaridis J, Angman L, Colonna M. Cutting edge: Human myeloid cells express an activating ILT receptor (ILT1) that associates with Fc receptor γ-chain. J Immunol. 1999;162:5-8. (In vitro control)
23.	Milbauer LC, Enenstein JA, Roney M, Solovey A, Bodempudi V, Nichols TC, et al. Blood outgrowth endothelial cell migration and trapping in vivo: a window into gene therapy. Transl Res. 2009;153:179-89. (In vitro control)
24.	Gibb DR, Shikh ME, Kang D, Rowe WJ, El Sayed R, Cichy J, et al. ADAM10 is essential for Notch2-dependent marginal zone B cell development and CD23 cleavage in vivo. J Exp Med. 2010;207:623-35. (In vitro control)
25.	Xu G, Wen X, Hong Y, Du H, Zhang X, Song J, et al. An anti-transferrin receptor antibody enhanced the growth inhibitory effects of chemotherapeutic drugs on human glioma cells. Int Immunopharmacol. 2011;11:1844-9. (In vitro control)
26.	Phoolcharoen W, Bhoo SH, Lai H, Ma J, Arntzen CJ, Chen Q, et al. Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana. Plant Biotechnol J. 2011;9:807-16. (Size exclusion chromatography control). (Size exclusion chromatography control)

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Mouse IgG-APC/CY7