The Antibody Company - SouthernBiotech
 
   

Rabbit IgG-BIOT

Cat. No.
0111-08  
Size
0.5 mg  
Price (USD)
$55.00 
Qty

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Source Normal rabbit serum
Format/Conjugate BIOT (Biotin)
Buffer Formulation Supplied in PBS/NaN3
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
Flow Cytometry 4-10
ELISA 1-3
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 4,11
Immunohistochemistry-Paraffin Sections 12-15
Immunohistochemistry-Whole Mount 23
Immunocytochemistry 16
Immunoblotting 21
Immunprecipitation 24,25
Chromatin Immunoprecipitation 25-28
Microarray 17,18
Blocking 19
In vitro Control 20
In vivo Control 29
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.

Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
 
References
1. Wimer-Mackin S, Hinchcliffe M, Petrie CR, Warwood SJ, Tino WT, Williams MS, et al. An intranasal vaccine targeting both the Bacillus anthracis toxin and bacterium provides protection against aerosol spore challenge in rabbits. Vaccine. 2006;24:3953-63. (ELISA)
2. Vaine M, Wang S, Crooks ET, Jiang P, Montefiori DC, Binley J, et al. Improved induction of antibodies against key neutralizing epitopes by human immunodeficiency virus type 1 gp120 DNA prime-protein boost vaccination compared to gp120 protein-only vaccination. J Virol. 2008;82:7369-78. (ELISA)
3. Vaine M, Wang S, Hackett A, Arthos J, Lu S. Antibody responses elicited through homologous or heterologous prime-boost DNA and protein vaccinations differ in functional activity and avidity. Vaccine. 2010;28:2999-3007. (ELISA)
4. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (FC, IHC-FS)
5. Cosmi L, Liotta F, Lazzeri E, Francalanci M, Angeli R, Mazzinghi B, et al. Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood. 2003;102:4107-14. (FC)
6. Zbytek B, Wortsman J, Slominski A. Characterization of a ultraviolet B-induced corticotropin-releasing hormone-proopiomelanocortin system in human melanocytes. Mol Endocrinol. 2006;20:2539-47. (FC)
7. Chu VT, Fröhlich A, Steinhauser G, Scheel T, Roch T, Fillatreau S, et al. Eosinophils are required for the maintenance of plasma cells in the bone marrow. Nat Immunol. 2011;12:151-60. (FC)
8. Zhu Z, Li R, Li H, Zhou T, Davis RS. FCRL5 exerts binary and compartment-specific influence on innate-like B-cell receptor signaling. Proc Natl Acad Sci USA. 2013;110:E1282-90. (FC)
9. Esposito L, Hunter KM, Clark J, Rainbow DB, Stevens H, Denesha J, et al. Investigation of soluble and transmembrane CTLA-4 isoforms in serum and microvesicles. J Immunol. 2014;193:889-900. (FC)
10. Jarman ER, Khambata VS, Ye LY, Cheung K, Thomas M, Duggan N, et al. A translational preclinical model of interstitial pulmonary fibrosis and pulmonary hypertension: mechanistic pathways driving disease pathophysiology. Physiol Rep. 2014;2(9):e12133. (FC)
11. Nesse W, Westra J, van der Wal JE, Abbas F, Nicholas AP, Vissink A, et al. The periodontium of periodontitis patients contains citrullinated proteins which may play a role in ACPA (anti-citrullinated protein antibody) formation. J Clin Periodontol. 2012;39:599-607. (IHC-FS)
12. Rehm S, Thomas RA, Smith KS, Mirabile RC, Gales TL, Eustis SL, et al. Novel vascular lesions in mice given a non-peptide vitronectin receptor antagonist. Toxicol Pathol. 2007;35:958-71. (IHC-PS)
13. Payne SC, Han JK, Huyett P, Negri J, Kropf EZ, Borish L, et al. Microarray analysis of distinct gene transcription profiles in non-eosinophilic chronic sinusitis with nasal polyps. Am J Rhinol. 2008;22:568-81. (IHC-PS)
14. Dalmas DA, Scicchitano MS, Chen Y, Kane J, Mirabile R, Schwartz LW, et al. Transcriptional profiling of laser capture microdissected rat arterial elements: fenoldopam-induced vascular toxicity as a model system. Toxicol Pathol. 2008;36:496-519. (IHC-PS)
15. Clancy RM, Alvarez D, Komissarova E, Barrat FJ, Swartz J, Buyon JP. Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block. J Immunol. 2010;184:2148-55. (IHC-PS)
16. Huizinga R, van der Star BJ, Kipp M, Jong R, Gerritsen W, Clarner T, et al. Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis. Glia. 2012;60:422-31. (ICC)
17. Fang X, Yu W, Li L, Shao J, Zhao N, Chen Q, et al. ChIP-seq and functional analysis of the SOX2 gene in colorectal cancers. OMICS. 2010;14:369-84. (Microarray)
18. Chen Y, Chen J, Loo A, Jaeger S, Bagdasarian L, Yu J, et al. Targeting HSF1 sensitizes cancer cells to HSP90 inhibition. Oncotarget. 2013;4:816-29. (Microarray)
19. Kapoor VN, Shin HM, Cho OH, Berg LJ, Kang J, Welsh RM. Regulation of tissue-dependent differences in CD8+ T cell apoptosis during viral infection. J Virol. 2014;88:9490-503. (Block)
20. Jacob AG, Singh RK, Mohammad F, Bebee TW, Chandler DS. The splicing factor FUBP1 is required for the efficient splicing of oncogene MDM2 pre-mRNA. J Biol Chem. 2014;289:17350-64. (In vitro control)
21. SouthernBiotech published data (WB)
22. Tada S, Yasui T, Nakatsuji Y, Okuno T, Koda T, Mochizuki H, et al. BAFF controls neural cell survival through BAFF receptor. PLoS One. 2013;8(7):e70924. (IHC-FS)
23. Lämmermann T, Afonso PV, Angermann BR, Wang JM, Kastenmüller W, Parent CA, et al. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature. 2013;498:371-5. (IHC-WM)
24. Ouchida R, Lu Q, Liu J, Li Y, Chu Y, Tsubata T, et al. FcμR interacts and cooperates with the B cell receptor To promote B cell survival. J Immunol. 2015;194:3096-101. (IP)
25. Power D, Santoso N, Dieringer M, Yu J, Huang H, Simpson S, et al. IFI44 suppresses HIV-1 LTR promoter activity and facilitates its latency. Virology. 2015;481:142-50. (IP, ChIP)
26. Wang Q, Williamson M, Bott S, Brookman-Amissah N, Freeman A, Nariculam J, et al. Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer. Oncogene. 2007;26:6560-5. (ChIP)
27. Repetny KJ, Zhong X, Holodick NE, Rothstein TL, Hansen U. Binding of LBP-1a to specific immunoglobulin switch regions in vivo correlates with specific repression of class switch recombination. Eur J Immunol. 2009;39:1387-94. (ChIP)
28. Tao Y, Sharif N, Zeng B, Cai Y, Guo Y. Lateral ventricle injection of orexin-A ameliorates central precocious puberty in rat via inhibiting the expression of MEG3. Int J Clin Exp Pathol. 2015;8:12564-70. (ChIP)
29. Woods PS, Tazi MF, Chesarino NM, Amer AO, David IC. TGF-β-induced IL-6 prevents development of acute lung injury in influenza A virus-infected F508del CFTR-heterozygous mice. Am J Physiol Lung Cell Mol Physiol. 2015;308:L1136-44. (In vivo control)
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