FLISA plate was coated with Goat Anti-Rabbit IgG Fab-UNLB (SB Cat. No. 4040-01) and Goat Anti-Rabbit IgG Fc-UNLB (SB Cat. No. 4041-01). Serially diluted Rabbit F(ab’)2 IgG-FITC (SB Cat. No. 0112-02) was captured and fluorescence intensity quantified.