Cat. No.
0116-13

Size
0.1 mg

Price (USD)
$167.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone	KLH/G1-2-2
Isotype	Rat IgG₁κ
Specificity	KLH
Format/Conjugate	SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light; Do not freeze Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - Flow Cytometry – Quality tested ^1-3 ELISA – Quality tested FLISA – Quality tested Immunocytochemistry – Reported in literature ^3,4 Blocking – Reported in literature ⁵ In vitro Control – Reported in literature ^6-8 In vivo Control – Reported in literature ^8-14
Recommended Dilutions	Please refer to product specific Technical Bulletin

FLISA plate was coated with Mouse Anti-Rat IgG1-UNLB (SB Cat. No. 3061-01), Mouse Anti-Rat IgG2a-UNLB (SB Cat. No. 3065-01), Mouse Anti-Rat IgG2b-UNLB (SB Cat. No. 3070-01), Mouse Anti-Rat IgG2c-UNLB (SB Cat. No. 3075-01), and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgG1-PE (SB Cat. No. 0116-09) was captured and fluorescence intensity quantified.

FLISA plate was coated with Mouse Anti-Rat IgG1-UNLB (SB Cat. No. 3061-01), Mouse Anti-Rat IgG2a-UNLB (SB Cat. No. 3065-01), Mouse Anti-Rat IgG2b-UNLB (SB Cat. No. 3070-01), Mouse Anti-Rat IgG2c-UNLB (SB Cat. No. 3075-01), and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgG1-PE (SB Cat. No. 0116-09) was captured and fluorescence intensity quantified.

References

1.	Chu VT, Fröhlich A, Steinhauser G, Scheel T, Roch T, Fillatreau S, et al. Eosinophils are required for the maintenance of plasma cells in the bone marrow. Nat Immunol. 2011;12:151-60. (FC)
2.	Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, et al. SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239. (FC)
3.	Gaspar-Elsas MI, Queto T, Vasconcelos Z, Jones CP, Lannes-Vieira J, Xavier-Elsas P. Evidence for a regulatory role of α4-integrins in the maturation of eosinophils generated from the bone marrow in the presence of dexamethasone. Clin Exp Allergy. 2009;39:1187-98. (FC, ICC)
4.	Chu VT, Berek C . Immunization induces activation of bone marrow eosinophils required for plasma cell survival. Eur J Immunol. 2012;42:130-7. (ICC)
5.	Talkington J, Nickell SP. Role of Fc gamma receptors in triggering host cell activation and cytokine release by Borrelia burgdorferi. Infect Immun. 2001;69:413-9. (Block)
6.	Lopez JI, Camenisch TD, Stevens MV, Sands BJ, McDonald J, Schroeder JA. CD44 attenuates metastatic invasion during breast cancer progression. Cancer Res. 2005;65:6755-63. (In vitro control)
7.	Lee S, Choi H, Eun S, Fukuyama S, Croft M. Nitric oxide modulates TGF-β-directive signals to suppress Foxp3⁺ regulatory T cell differentiation and potentiate Th1 development. J Immunol. 2011;186:6972-80. (In vitro control)
8.	Brouxhon SM, Kyrkanides S, Teng X, Raja V, O'Banion MK, Clarke R, et al. Monoclonal antibody against the ectodomain of E-cadherin (DECMA-1) suppresses breast carcinogenesis: involvement of the HER/PI3K/Akt/mTOR and IAP pathways. Clin Cancer Res. 2013;19:3234-46. (In vitro control, in vivo control)
9.	Haeryfar SM, DiPaolo RJ, Tscharke DC, Bennink JR, Yewdell JW. Regulatory T cells suppress CD8⁺ T cell responses induced by direct priming and cross-priming and moderate immunodominance disparities. J Immunol. 2005;174:3344-51. (In vivo control)
10.	Liang Y, Christopher K, Finn PW, Colson YL, Perkins DL. Graft produced interleukin-6 functions as a danger signal and promotes rejection after transplantation. Transplantation. 2007;84:771-7. (In vivo control)
11.	Dakhama A, Lee Y, Ohnishi H, Jing X, Balhorn A, Takeda K, et al. Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice. J Allergy Clin Immunol. 2009;123:138-45. (In vivo control)
12.	Zhang Z, Li Z, Yan S, Wang X, Abecassis M. TNF-α signaling is not required for in vivo transcriptional reactivation of latent murine cytomegalovirus. Transplantation. 2009;88:640-5. (In vivo control)
13.	Saha S, Jing X, Park SY, Wang S, Li X, Gupta D. Peptidoglycan recognition proteins protect mice from experimental colitis by promoting normal gut flora and preventing induction of interferon-γ. Cell Host Microbe. 2010;8:147-62. (In vivo control)
14.	Brouxhon SM, Kyrkanides S, Raja V, Silberfeld A, Teng X, Trochesset D, et al. Ectodomain-specific E-cadherin antibody suppresses skin SCC growth and reduces tumor grade: a multitargeted therapy modulating RTKs and the PTEN-p53-MDM2 axis. Mol Cancer Ther. 2014;13:1791-802. (In vivo control)

Spectral Red^® is a registered trademark of Southern Biotechnology Associates, Inc.
Spectral Red^® is a PE/CY5 tandem conjugate
Cy^® is a registered trademark of GE Healthcare

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Rat IgG1-SPRD

Rat IgG₁-SPRD