ApoScreen® Annexin V Apoptosis Kit-FITC

Cat. No.
10010-02  
Size
100 tests   		Price (USD)
$260.00 
Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Description ApoScreen® Annexin V Apoptosis Kit-FITC (SB Cat. No. 10010-02) employs a fluorescein-conjugated Annexin V (Annexin V-FITC) in concert with propidium iodide (PI) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis, cells begin to display phosphatidylserine (PS) on the outer cell membrane where it is readily detectable by staining the cells with Annexin V-FITC. As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, PI can readily move across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lost membrane integrity through mechanisms including necrosis. When cells are double stained with Annexin V-FITC and PI, three different cell populations may be observed - (i) live cells that do not stain with either Annexin V-FITC or PI; (ii) necrotic cells that stain with both reagents; and (iii) apoptotic cells that stain with Annexin V-FITC only. Analysis may be performed on any flow cytometer equipped with a single laser using excitation at 488 nm. Alternatively, the cells can be examined with a fluorescence microscope equipped with filters for discrimination of FITC and rhodamine/PI.
Kit Components Annexin V-FITC - 1.0 mL
Propidium Iodide (PI) - 1.0 mL
Annexin V Binding Buffer 10X - 7 x 1.5 mL
Buffer Formulation Annexin V-FITC - Annexin V Binding Buffer
PI - Annexin V Binding Buffer
Annexin V Binding Buffer - Contains 0.1% sodium azide and proprietary ingredients
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications Flow Cytometry – Quality tested 1-7, 9-34
Immunocytochemistry – Reported in literature 6,8,35
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ApoScreen® Annexin V Apoptosis Kit-FITC
    Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-FITC (SB Cat. No. 10010-02).

Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-FITC (SB Cat. No. 10010-02).
 
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References
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ApoScreen® is a registered trademark of Southern Biotechnology Associates, Inc.