The Antibody Company - SouthernBiotech
 
   

ApoScreen® Annexin V-UNLB

Cat. No.
10040-01  
Size
0.1 mg  
Price (USD)
$130.00 
Qty
Add to Cart
Description ApoScreen® Annexin V (SB Cat. No. 10040) is a recombinant protein that is expressed in E. coli. A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface. Annexin V, a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for phosphatidylserine (PS)-containing phospholipid bilayers. Conjugated Annexin V can be used to monitor changes in cell membrane phospholipid asymmetry, thereby providing a convenient tool for detection of apoptotic cells.
Source E. coli
Buffer Formulation Supplied lyophilized from phosphate buffered saline
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications Flow Cytometry – Quality tested 1-25
Immunohistochemistry-Whole Mount – Reported in literature 26
Immunocytochemistry – Reported in literature 6,27
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Day old BALB/c mouse bone marrow cells were stained with Annexin V-CY5 (SB Cat. No. 10040-15) with and without Propidium Iodide (SB Cat. No. 10044-01).

Day old BALB/c mouse bone marrow cells were stained with Annexin V-CY5 (SB Cat. No. 10040-15) with and without Propidium Iodide (SB Cat. No. 10044-01).
 
References
1. Gao-Uozumi C, Uozumi N, Miyoshi E, Nagai K, Ikeda Y, Teshima T, et al. A novel carbohydrate binding activity of annexin V toward a bisecting N-acetylglucosamine. Glycobiology. 2000;10:1209-16. (FC)
2. Rademaekers A, Kölsch E, Specht C. T cell mediated antibody invariance in an immune response against a bacterial carbohydrate antigen requires CD28/B7-1 costimulation. Dev Immunol. 2001;8:243-57. (FC)
3. Kunz-Schughart LA, Schroeder JA, Wondrak M, van Rey F, Lehle K, Hofstaedter F, et al. Potential of fibroblasts to regulate the formation of three-dimensional vessel-like structures from endothelial cells in vitro. Am J Physiol Cell Physiol. 2006;290:C1385-98. (FC)
4. Woolf E, Brenner O, Goldenberg D, Levanon D, Groner Y. Runx3 regulates dendritic epidermal T cell development. Dev Biol. 2007;303:703-14. (FC)
5. Saddler C, Ouillette P, Kujawski L, Shangary S, Talpaz M, Kaminski M, et al. Comprehensive biomarker and genomic analysis identifies p53 status as the major determinant of response to MDM2 inhibitors in chronic lymphocytic leukemia. Blood. 2008;111:1584-93. (FC)
6. Reif RD, Martinez MM, Wang K, Pappas D. Simultaneous cell capture and induction of apoptosis using an anti-CD95 affinity microdevice. Anal Bioanal Chem. 2009;395:787-95. (FC, ICC)
7. de Medina P, Payré B, Boubekeur N, Bertrand-Michel J, Tercé F, Silvente-Poirot S, et al. Ligands of the antiestrogen-binding site induce active cell death and autophagy in human breast cancer cells through the modulation of cholesterol metabolism. Cell Death Differ. 2009;16:1372-84. (FC)
8. Siegers GM, Dhamko H, Wang X, Mathieson AM, Kosaka Y, Felizardo TC, et al. Human Vδ1 γδ T cells expanded from peripheral blood exhibit specific cytotoxicity against B-cell chronic lymphocytic leukemia-derived cells. Cytotherapy. 2011;13:753-64. (FC)
9. Eckermann CW, Lehle K, Schmid SA, Wheatley DN, Kunz-Schughart LA. Characterization and modulation of fibroblast/endothelial cell co-cultures for the in vitro preformation of three-dimensional tubular networks. Cell Biol Int. 2011;35:1097-110. (FC)
10. Alberter B, Vogel B, Lengenfelder D, Full F, Ensser A. Genome-wide histone acetylation profiling of Herpesvirus saimiri in human T cells upon induction with a histone deacetylase inhibitor. J Virol. 2011;85:5456-64. (FC)
11. Gottleib Y, Topaz O, Cohen LA, Yakov LD, Haber T, Morgenstern A, et al. Physiologically aged red blood cells undergo erythrophagocytosis in vivo but not in vitro. Haematologica. 2012;97:994-1002. (FC)
12. Di Pardo A, Amico E, Favellato M, Castrataro R, Fucile S, Squitieri F, et al. FTY720 (fingolimod) is a neuroprotective and disease-modifying agent in cellular and mouse models of Huntington disease. Hum Mol Genet. 2014;23:2251-65. (FC)
13. Liyanage NP, Dassanayake RP, Kuszynski CA, Duhamel GE. Contribution of Helicobacter hepaticus cytolethal distending toxin subunits to human epithelial cell cycle arrest and apoptotic death in vitro. Helicobacter. 2013;18:433-43. (FC)
14. Jenks SA, Palmer E, Marin E, Hartson L, Chida AS, Richardson C, et al. 9G4+ autoantibodies are an important source of apoptotic cell reactivity associated with high levels of disease activity in systemic lupus erythematosus. Arthritis Rheum. 2013;65:3165-75. (FC)
15. Vogel B, Tennert K, Full F, Ensser A. Efficient generation of human natural killer cell lines by viral transformation. Leukemia. 2014;28:192-5. (FC)
16. Huang S, Wu B, Li D, Zhou W, Deng G, Zhang K, et al. Knockdown of astrocyte elevated gene-1 inhibits tumor growth and modifies microRNAs expression profiles in human colorectal cancer cells. Biochem Biophys Res Commun. 2014;444:338-45. (FC)
17. Peres RS, Liew FY, Talbot J, Carregaro V, Oliveira RD, Almeida SL, et al. Low expression of CD39 on regulatory T cells as a biomarker for resistance to methotrexate therapy in rheumatoid arthritis. Proc Natl Acad Sci USA. 2015;112:2509-14. (FC)
18. Meng Q, Wang H, Song Z, Shan Z, Yuan Z, Zheng Q, et al. Overexpression of NDC80 is correlated with prognosis of pancreatic cancer and regulates cell proliferation. Am J Cancer Res. 2015;5:1730-40. (FC)
19. Koh DW, Powell DP, Blake SD, Hoffman JL, Hopkins MM, Feng X. Enhanced cytotoxicity in triple-negative and estrogen receptor-positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel. Oncol Rep. 2015;34:1589-98. (FC)
20. Jia A, Lv Y, Guo X, Ren L, Qin J. Ectopic expression of p33ING1b suppresses proliferation and induces apoptosis in colonic adenocarcinoma cells. Oncol Lett. 2015;10:1517-22. (FC)
21. Squitieri F, Di Pardo A, Favellato M, Amico E, Maglione V, Frati L. Pridopidine, a dopamine stabilizer, improves motor performance and shows neuroprotective effects in Huntington disease R6/2 mouse model. J Cell Mol Med. 2015;19:2540-8. (FC)
22. Rancan C, Schirrmann L, Hüls C, Zeidler R, Moosmann A. Latent membrane protein LMP2A impairs recognition of EBV-infected cells by CD8+ T cells. PLoS Pathog. 2015;11(6):e1004906. (FC)
23. Meusch U, Klingner M, Mathar C, Malysheva O, Baerwald C, Rossol M, et al. Brief report: Autocrine cytokine-mediated deficiency of TRAIL-induced monocyte apoptosis in rheumatoid arthritis. Arthritis Rheumatol. 2015;67:1760-5. (FC)
24. Batra SA, Shanthalingam S, Munske GR, Raghavan B, Kugadas A, Bavanthasivam J, et al. Acylation enhances, but is not required for, the cytotoxic activity of Mannheimia haemolytica leukotoxin in bighorn sheep. Infect Immun. 2015;83:3982-8. (FC)
25. Betancourt D, Ramos JC, Barber GN. Retargeting oncolytic vesicular stomatitis virus to human T-cell lymphotropic virus type 1-associated adult T-cell leukemia. J Virol. 2015;89:11786-800. (FC)
26. Bourges J, Valamanesh F, Torriglia A, Jeanny J, Savoldelli M, Renard G, et al. Cornea graft endothelial cells undergo apoptosis by way of an alternate (caspase-independent) pathway. Transplantation. 2004;78:316-23. (IHC-WM)
27. Polak MB, Valamanesh F, Felt O, Torriglia A, Jeanny J, Bourges J, et al. Controlled delivery of 5-chlorouracil using poly(ortho esters) in filtering surgery for glaucoma. Invest Ophthalmol Vis Sci. 2008;49:2993-3003. (ICC)
ApoScreen® is a registered trademark of SouthernBiotechnology Associates, Inc.
Back to Product List
Customer & Technical Service 800.722.2255 (USA & Canada) . 205.945.1774 . Fax 205.945.8768
Copyright © 2017 SouthernBiotech
Follow SouthernBiotech