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Mouse Anti-Bax-UNLB

Cat. No.
10060-01  
Size
0.1 mg  
Price (USD)
$180.00 
Qty
Add to Cart
Clone 6A7
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen Bax peptide (a.a. 12-24) common to mouse, rat, and human
Specificity Mouse/Rat/Human/Monkey/Bovine Bax
Alternalte Name(s) Apoptosis regulator BAX, BCL2 associated X protein
Description Bax is a 20-22 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. In murine thymocytes, it is expressed primarily as a cytoplasmic protein. Over-expression of Bax promotes apoptosis by formation of homodimers and through heterodimerization with Bcl-2, an inhibitor of apoptosis. The monoclonal antibody 6A7 reacts with human, mouse and rat Bax but does not bind the soluble cytosolic form of Bax; however, treatment of cells with non-ionic detergents exposes the epitope and allows binding of 6A7 to monomeric forms of Bax but not Bax complexed with either Bcl-2 or Bcl-xL.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Immunoblotting – Quality tested 2-5
Immunoprecipitation – Reported in literature 1,2,6,7
Flow Cytometry – Reported in literature 7-9
Immunohistochemistry-Frozen Sections – Reported in literature 4
Immunohistochemistry-Paraffin Sections – Reported in literature 5,10-12
Immunocytochemistry – Reported in literature 3,7-9,12-15
Electron Microscopy – Reported in literature 14,16
Neutralization – Reported in literature 17
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bax-UNLB (SB Cat. No. 10060-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.

Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bax-UNLB (SB Cat. No. 10060-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
 
References
1. Hsu Y, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997;272:13829-34. (Immunogen, IP)
2. Li B, Dou QP. Bax degradation by the ubiquitin-proteasome-dependent pathway- Involvement in tumor survival and progression. Proc Natl Acad Sci USA. 2000;97:3850-5. (WB, IP)
3. Melino G, Bernassola F, Ranalli M, Yee K, Zong WX, Corazzari M, et al. p73 Induces apoptosis via PUMA transactivation and Bax mitochondrial translocation. J Biol Chem. 2004;279:8076-83. (WB, ICC)
4. Utsunomiya K, Watanabe J, Takamori Y, Kataoka Y, Kurokawa K, Yamada H. Crossreaction with an anti-Bax antibody reveals novel multi-endocrine cellular antigen. J Histochem Cytochem. 2004;52:805-12. (WB, IHC-FS)
5. Kudo W, Lee H, Smith MA, Zhu X, Matsuyama S, Lee H. Inhibition of Bax protects neuronal cells from oligomeric Aβ neurotoxicity. Cell Death Dis. 2012;3:e309. (WB, IHC-PS)
6. Hsu Y, Youle RJ. Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations. J Biol Chem. 1998;273:10777-83. (IP)
7. Cheng S, Gao N, Zhang Z, Chen G, Budhraja A, Ke Z, et al. Quercetin induces tumor-selective apoptosis through downregulation of Mcl-1 and activation of Bax. Clin Cancer Res. 2010;16:5679-91. (IP, FC, ICC)
8. Bellosillo B, Villamor N, López-Guillermo A, Marcé S, Bosch F, Campo E, et al. Spontaneous and drug-induced apoptosis is mediated by conformational changes of Bax and Bak in B-cell chronic lymphocytic leukemia. Blood. 2002;100:1810-6. (FC, ICC)
9. Cabon L, Galán-Malo P, Bouharrour A, Delavallée L, Brunelle-Navas M, Lorenzo HK, et al. BID regulates AIF-mediated caspase-independent necroptosis by promoting BAX activation. Cell Death Differ. 2012;19:245-56. (FC, ICC)
10. Saatli B, Kizildag S, Posaci C, Dogan E, Koyuncuoglu M, Ulukus EC, et al. Long-term effects of GnRH agonist, GnRH antagonist, and estrogen plus progesterone treatment on apoptosis related genes in rat ovary. Fertil Steril. 2009;91:2006-11. (IHC-PS)
11. Wang X, Han W, Du X, Zhu C, Carlsson Y, Mallard C, et al. Neuroprotective effect of Bax-inhibiting peptide on neonatal brain injury. Stroke. 2010;41:2050-5. (IHC-PS)
12. Zaman F, Chrysis D, Huntjens K, Fadeel B, Sävendahl L. Ablation of the pro-apoptotic protein Bax protects mice from glucocorticoid-induced bone growth impairment. PLoS One. 2012;7(3):e33168. (IHC-PS, ICC)
13. Dewson G, Cohen GM, Wardlaw AJ. Interleukin-5 inhibits translocation of Bax to the mitochondria, cytochrome c release, and activation of caspases in human eosinophils. Blood. 2001;98:2239-47. (ICC)
14. Karbowski M, Lee Y, Gaume B, Jeong S, Frank S, Nechushtan A, et al. Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis. J Cell Biol. 2002;159:931-8. (ICC, EM)
15. Thomenius MJ, Wang NS, Reineks EZ, Wang Z, Distelhorst CW. Bcl-2 on the endoplasmic reticulum regulates Bax activity by binding to BH3-only proteins. J Biol Chem. 2003;278:6243-50. (ICC)
16. Nechushtan A, Smith CL, Lamensdorf I, Yoon S, Youle RJ. Bax and Bak coalesce into novel mitochondria-associated clusters during apoptosis. J Cell Biol. 2001;153:1265-76. (EM)
17. Zhang Y, McLaughlin R, Goodyer C, LeBlanc A. Selective cytotoxicity of intracellular amyloid beta peptide1-42 through p53 and Bax in cultured primary human neurons. J Cell Biol. 2002;156:519-29. (Neut)
18. Hsu Y, Smaili S. Molecular characterization of the proapoptotic protein Bax. In: LeBlanc AC, editor. Apoptosis Techniques and Protocols. Totowa: Humana Press; 2002. p. 1-20. (Monkey & Bovine Reactivity)
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