Rat Anti-Human GM-CSF-UNLB

Cat. No.
10111-01  
Size
0.5 mg   		Price (USD)
$240.00 
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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone BVD2-23B6
Isotype Rat IgG2aκ
Isotype Control Rat IgG2a-UNLB
Immunogen E. coli-expressed human GM-CSF
Specificity Human/Rhesus/Cynomolgus GM-CSF
Alternalte Name(s) Granulocyte/macrophage colony-stimulating factor, CSF-α, pluripoietin-α, eosinophil colony stimulating factor, Eo-CSF, burst promoting activity, BPA
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Capture – Quality tested 1-7
ELISpot-Capture – Reported in literature 4,6,8
Immunoprecipitation – Reported in literature 3
Neutralization – Reported in literature 1,3,9,10
Multiplex-Capture – Reported in literature 11

Note – May be paired with the biotinylated clone BVD2-21C11 (SB Cat. No. 10112-08) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794135
Gene ID
1437 (Human)
574371 (Rhesus)
102118747 (Cynomolgus)
Gene Symbol
CSF2 (Human)
CSF2 (Rhesus)
CSF2 (Cynomolgus)
Gene Aliases CSF; GMCSF; GM-CSF
Uniprot ID
P04141 (Human)
Uniprot Name
CSF2_HUMAN 
  • Rat Anti-Human GM-CSF-UNLB
    Standard curve generated with Rat Anti-Human GM-CSF-UNLB (SB Cat. No. 10111-01; Clone BVD2-23B6) and Rat Anti-Human GM-CSF-BIOT (SB Cat. No. 10112-08; Clone BVD2-21C11) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Human GM-CSF-UNLB (SB Cat. No. 10111-01; Clone BVD2-23B6) and Rat Anti-Human GM-CSF-BIOT (SB Cat. No. 10112-08; Clone BVD2-21C11) followed by Streptavidin-HRP (SB Cat. No. 7100-05)
 
Related Products (4)
References
1. Abrams JS, Silver JE, Van Dyke RE, Gleich GJ. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GM-CSF. In: Gleich GJ, Kay AB, editors. Eosinophils in allergy and inflammation. New York: Marcel Dekker; 1994. p. 133-57. (Immunogen, ELISA-Capture, Neut)
2. Bacchetta R, de Waal Malefijt R, Yssel H, Abrams J, de Vries JE, Spits H, et al. Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-γ and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990;144:902-8. (ELISA-Capture)
3. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, IP, Neut)
4. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Capture, ELISPOT-Capture)
5. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
6. Mäkitalo B, Andersson M, Areström I, Karlén K, Villinger F, Ansari A, et al. ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques. J Immunol Methods. 2002;270:85-97. (ELISA-Capture, ELISPOT-Capture, Rhesus & Cynomolgus Reactivity)
7. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Capture)
8. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
9. Denburg JA, Silver JE, Abrams JS. Interleukin-5 is a human basophilopoietin: induction of histamine content and basophilic differentiation of HL-60 cells and of peripheral blood basophil-eosinophil progenitors. Blood. 1991;77:1462-8. (Neut)
10. Kelly EA, Koziol-White CJ, Clay KJ, Liu LY, Bates ME, Bertics PJ, et al. Potential contribution of IL-7 to allergen-induced eosinophilic airway inflammation in asthma. J Immunol. 2009;182:1404-10. (Neut)
11. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Capture, Cynomolgus Reactivity)