Rat Anti-Human IL-5-LE/AF

Cat. No.
10119-14  
Size
0.5 mg   		Price (USD)
$250.00 
Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone JES1-39D10
Isotype Rat IgG2aκ
Isotype Control Rat IgG2a-LE/AF
Immunogen COS-expressed human IL-5
Specificity Human IL-5
Alternalte Name(s) Interleukin-5, eosinophil differentiation factor, EDF, T cell replacing factor-1, TRF-1, eosinophil colony stimulating factor, Eo-CSF, B cell growth factor-2, BCGF-2, B cell differentiation factor for IgM, BCDF-m, IgA enhancing factor, IgA-EF
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling -20°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Capture – Quality tested 1-7
ELISA-Detection – Quality tested
ELISpot-Capture – Reported in literature 4,8
Flow Cytometry – Reported in literature 9,10
Immunohistochemistry-Frozen Sections – Reported in literature 11,12
Immunocytochemistry – Reported in literature 9
Neutralization – Reported in literature 1,3,13
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794156
Gene ID
3567 (Human)
Gene Symbol
IL5 (Human)
Gene Aliases EDF; IL-5; TRF
Uniprot ID
P05113  (Human)
Uniprot Name
IL5_HUMAN 
  • Rat Anti-Human IL-5-BIOT
    Standard curve generated with Rat Anti-Human IL-5-UNLB (SB Cat. No. 10118-01; Clone JES1-5A10) and Rat Anti-Human IL-5-BIOT (SB Cat. No. 10119-08; Clone JES1-39D10) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Human IL-5-UNLB (SB Cat. No. 10118-01; Clone JES1-5A10) and Rat Anti-Human IL-5-BIOT (SB Cat. No. 10119-08; Clone JES1-39D10) followed by Streptavidin-HRP (SB Cat. No. 7100-05)
 
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References
1. Abrams JS, Silver JE, Van Dyke RE, Gleich GJ. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GM-CSF. In: Gleich GJ, Kay AB, editors. Eosinophils in allergy and inflammation. New York: Marcek Dekker; 1994. p. 133-57. (Immunogen, ELISA-Capture, Neut)
2. Bacchetta R, de Waal Malefijt R, Yssel H, Abrams J, de Vries JE, Spits H, et al. Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-γ and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990;144:902-8. (ELISA-Capture)
3. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
4. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Capture, ELISPOT-Capture)
5. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
6. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Capture)
7. Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (ELISA-Capture)
8. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
9. Krouwels FH, Nocker RE, Snoek M, Lutter R, van der Zee JS, Weller FR, et al. Immunocytochemical and flow cytofluorimetric detection of intracellular IL-4, IL-5 and IFN-γ: applications using blood- and airway-derived cells. J Immunol Methods. 1997;203:89-101. (FC, ICC)
10. Gereda JE, Leung DY, Thatayatikom A, Streib JE, Price MR, Klinnert MD, et al. Relation between house-dust endotoxin exposure, type 1 T-cell development, and allergen sensitisation in infants at high risk of asthma. Lancet. 2000;355:1680-3. (FC)
11. Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
12. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
13. Denburg JA, Silver JE, Abrams JS. Interleukin-5 is a human basophilopoietin: induction of histamine content and basophilic differentiation of HL-60 cells and of peripheral blood basophil-eosinophil progenitors. Blood. 1991;77:1462-8. (Neut)