Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

Rat Anti-Human IL-5-BIOT (JES1-39D10)

Cat. No.:
10119-08
Biotin Anti-Human IL-5 antibody for use in flow cytometry, ELISA, and immunohistochemistry / immunocytochemistry assays.
$286.00
Size Price (USD) Quantity
0.5 mg $286.00
More Information
Clone JES1-39D10
Isotype Rat IgG2aκ
Isotype Control Rat IgG2a-BIOT (KLH/G2a-1-1)
Specificity Human IL-5
Alternative Names Interleukin-5, eosinophil differentiation factor, EDF, T cell replacing factor-1, TRF-1, eosinophil colony stimulating factor, Eo-CSF, B cell growth factor-2, BCGF-2, B cell differentiation factor for IgM, BCDF-m, IgA enhancing factor, IgA-EF
Immunogen COS-expressed human IL-5
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA-Capture – Quality tested 1-7
ELISA-Detection – Quality tested
ELISpot-Capture – Reported in literature 4,8
Flow Cytometry – Reported in literature 9,10
Immunohistochemistry-Frozen Sections – Reported in literature 11,12
Immunocytochemistry – Reported in literature 9
Neutralization – Reported in literature 1,3,13

RRID Number AB_2794155
Gene ID 3567 (Human)
Gene ID Symbol IL5 (Human)
Gene ID Aliases EDF; IL-5; TRF
UniProt ID P05113 (Human
UniProt Name IL5_HUMAN (Human)

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
or call 800.722.2255
  1. 1. Abrams JS, Silver JE, Van Dyke RE, Gleich GJ. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GM-CSF. In: Gleich GJ, Kay AB, editors. Eosinophils in allergy and inflammation. New York: Marcek Dekker; 1994. p. 133-57. (Immunogen, ELISA-Capture, Neut)
  2. 2. Bacchetta R, de Waal Malefijt R, Yssel H, Abrams J, de Vries JE, Spits H, et al. Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-γ and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990;144:902-8. (ELISA-Capture)
  3. 3. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
  4. 4. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Capture, ELISPOT-Capture)
  5. 5. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
  6. 6. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Capture)
  7. 7. Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (ELISA-Capture)
  8. 8. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
  9. 9. Krouwels FH, Nocker RE, Snoek M, Lutter R, van der Zee JS, Weller FR, et al. Immunocytochemical and flow cytofluorimetric detection of intracellular IL-4, IL-5 and IFN-γ: applications using blood- and airway-derived cells. J Immunol Methods. 1997;203:89-101. (FC, ICC)
  10. 10. Gereda JE, Leung DY, Thatayatikom A, Streib JE, Price MR, Klinnert MD, et al. Relation between house-dust endotoxin exposure, type 1 T-cell development, and allergen sensitisation in infants at high risk of asthma. Lancet. 2000;355:1680-3. (FC)
  11. 11. Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
  12. 12. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
  13. 13. Denburg JA, Silver JE, Abrams JS. Interleukin-5 is a human basophilopoietin: induction of histamine content and basophilic differentiation of HL-60 cells and of peripheral blood basophil-eosinophil progenitors. Blood. 1991;77:1462-8. (Neut)
See All References