Cat. No.
10120-01

Size
0.5 mg

Price (USD)
$200.00

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Technical Bulletin Certificate of
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Clone	MQ2-13A5
Isotype	Rat IgG₁κ
Isotype Control	Rat IgG₁-UNLB
Immunogen	COS-expressed human IL-6
Specificity	Human/Rhesus/Cynomolgus IL-6
Alternalte Name(s)	Interleukin-6, hepatocyte stimulating factor, HSF, hepatocyte growth factor, HGF, B cell stimulating factor-2, BSF-2, BSF2, cytotoxic T cell differentiation factor, CDF, hybridoma/plasmacytoma growth factor, HPGF
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA-Capture – Quality tested ^3,4 ELISA-Detection – Reported in literature ^1,2 ELISpot-Capture – Reported in literature ^3,5 Flow Cytometry – Reported in literature ^6,7 Immunoprecipitation – Reported in literature ⁸ Neutralization – Reported in literature ^1,9 Multiplex-Capture – Reported in literature ¹⁰ Note – May be paired with the biotinylated clone MQ2-39C3 (SB Cat. No. 10121-08) in a sandwich ELISA
Recommended Dilutions	Please refer to product specific Technical Bulletin

Standard curve generated with Rat Anti-Human IL-6-UNLB (SB Cat. No. 10120-01; Clone MQ2-13A5) and Rat Anti-Human IL-6-BIOT (SB Cat. No. 10121-08; Clone MQ2-39C3) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Human IL-6-UNLB (SB Cat. No. 10120-01; Clone MQ2-13A5) and Rat Anti-Human IL-6-BIOT (SB Cat. No. 10121-08; Clone MQ2-39C3) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

References

1.	Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
2.	Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
3.	Mäkitalo B, Andersson M, Areström I, Karlén K, Villinger F, Ansari A, et al. ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques. J Immunol Methods. 2002;270:85-97. (ELISA-Capture, ELISPOT-Capture, Rhesus & Cynomolgus Reactivity)
4.	Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Capture)
5.	Hansson T, Dannæus A, Klareskog L. Cytokine-producing cells in peripheral blood of children with coeliac disease secrete cytokines with a type 1 profile. Clin Exp Immunol. 1999;116:246-50. (ELISPOT-Capture)
6.	Keller ET, Zhang J, Yao Z, Qi Y. The impact of chronic estrogen deprivation on immunologic parameters in the ovariectomized rhesus monkey (Macaca mulatta) model of menopause. J Reprod Immunol. 2001;50:41-55. (FC, Rhesus Reactivity)
7.	Sestak K, McNeal MM, Choi A, Cole MJ, Ramesh G, Alvarez X, et al. Defining T-cell-mediated immune responses in rotavirus-infected juvenile rhesus macaques. J Virol. 2004;78:10258-64. (FC, Rhesus Reactivity)
8.	Zou J, Morford LA, Chougnet C, Dix AR, Brooks AG, Torres N, et al. Human glioma-induced immunosuppression involves soluble factor(s) that alters monocyte cytokine profile and surface markers. J Immunol. 1999;162:4882-92. (IP)
9.	Sharma S, Nemeth E, Chen Y, Goodnough J, Huston A, Roodman GD, et al. Involvement of hepcidin in the anemia of multiple myeloma. Clin Cancer Res. 2008;14:3262-7. (Neut)
10.	Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Capture, Cynomolgus Reactivity)

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