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Rat Anti-Human IL-6-UNLB

Cat. No.
10120-01  
Size
0.5 mg  
Price (USD)
$200.00 
Qty
Add to Cart
Clone MQ2-13A5
Isotype Rat IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen COS-expressed human IL-6
Specificity Human/Rhesus/Cynomolgus IL-6
Alternalte Name(s) Interleukin-6, hepatocyte stimulating factor, HSF, hepatocyte growth factor, HGF, B cell stimulating factor-2, BSF-2, BSF2, cytotoxic T cell differentiation factor, CDF, hybridoma/plasmacytoma growth factor, HPGF
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Capture – Quality tested 3,4
ELISA-Detection – Reported in literature 1,2
ELISpot-Capture – Reported in literature 3,5
Flow Cytometry – Reported in literature 6,7
Immunoprecipitation – Reported in literature 8
Neutralization – Reported in literature 1,9
Multiplex-Capture – Reported in literature 10

Note – May be paired with the biotinylated clone MQ2-39C3 (SB Cat. No. 10121-08) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Standard curve generated with Rat Anti-Human IL-6-UNLB (SB Cat. No. 10120-01; Clone MQ2-13A5) and Rat Anti-Human IL-6-BIOT (SB Cat. No. 10121-08; Clone MQ2-39C3) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Human IL-6-UNLB (SB Cat. No. 10120-01; Clone MQ2-13A5) and Rat Anti-Human IL-6-BIOT (SB Cat. No. 10121-08; Clone MQ2-39C3) followed by Streptavidin-HRP (SB Cat. No. 7100-05)
 
References
1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
2. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
3. Mäkitalo B, Andersson M, Areström I, Karlén K, Villinger F, Ansari A, et al. ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques. J Immunol Methods. 2002;270:85-97. (ELISA-Capture, ELISPOT-Capture, Rhesus & Cynomolgus Reactivity)
4. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Capture)
5. Hansson T, Dannæus A, Klareskog L. Cytokine-producing cells in peripheral blood of children with coeliac disease secrete cytokines with a type 1 profile. Clin Exp Immunol. 1999;116:246-50. (ELISPOT-Capture)
6. Keller ET, Zhang J, Yao Z, Qi Y. The impact of chronic estrogen deprivation on immunologic parameters in the ovariectomized rhesus monkey (Macaca mulatta) model of menopause. J Reprod Immunol. 2001;50:41-55. (FC, Rhesus Reactivity)
7. Sestak K, McNeal MM, Choi A, Cole MJ, Ramesh G, Alvarez X, et al. Defining T-cell-mediated immune responses in rotavirus-infected juvenile rhesus macaques. J Virol. 2004;78:10258-64. (FC, Rhesus Reactivity)
8. Zou J, Morford LA, Chougnet C, Dix AR, Brooks AG, Torres N, et al. Human glioma-induced immunosuppression involves soluble factor(s) that alters monocyte cytokine profile and surface markers. J Immunol. 1999;162:4882-92. (IP)
9. Sharma S, Nemeth E, Chen Y, Goodnough J, Huston A, Roodman GD, et al. Involvement of hepcidin in the anemia of multiple myeloma. Clin Cancer Res. 2008;14:3262-7. (Neut)
10. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Capture, Cynomolgus Reactivity)
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