Rat Anti-Human IL-7-LE/AF

Cat. No.
10122-14  
Size
0.5 mg   		Price (USD)
$280.00 
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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone BVD10-40F6
Isotype Rat IgG1κ
Isotype Control Rat IgG1-LE/AF
Immunogen E. coli-expressed human IL-7
Specificity Human IL-7
Alternalte Name(s) Interleukin-7, lymphopoietin-1, LP-1, Lpo-1, pre-B cell growth factor, PBGF, thymocyte growth factor
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; handle under aseptic conditions
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Capture – Quality tested 1-3
Immunocytochemistry – Reported in literature 4
Neutralization – Reported in literature 1,5

Note – May be paired with the biotinylated clone BVD10-11C10 (SB Cat. No. 10123-08) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794173
Gene ID
3574 (Human)
Gene Symbol
IL7 (Human)
Gene Aliases IL-7
Uniprot ID
P13232 (Human)
Uniprot Name
IL7_HUMAN 
  • Rat Anti-Human IL-7-UNLB
    Standard curve generated with Rat Anti-Human IL-7-UNLB (SB Cat. No. 10122-01; Clone BVD10-40F6) and Rat Anti-Human IL-7-BIOT (SB Cat. No. 10123-08; Clone BVD10-11C10) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)

Standard curve generated with Rat Anti-Human IL-7-UNLB (SB Cat. No. 10122-01; Clone BVD10-40F6) and Rat Anti-Human IL-7-BIOT (SB Cat. No. 10123-08; Clone BVD10-11C10) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)
 
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References
1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24.(ELISA-Capture, Neut)
2. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
3. Kelly EA, Koziol-White CJ, Clay KJ, Liu LY, Bates ME, Bertics PJ, et al. Potential contribution of IL-7 to allergen-induced eosinophilic airway inflammation in asthma. J Immunol. 2009;182:1404-10. (ELISA-Capture)
4. Bazdar DA, Kalinowska M, Panigrahi S, Sieg SF. Recycled IL-7 can be delivered to neighboring T cells. J Immunol. 2015;194:4698-704. (ICC)
5. Moreau I, Duvert V, Caux C, Galmiche MC, Charbord P, Banchereau J, et al. Myofibroblastic stromal cells isolated from human bone marrow induce the proliferation of both early myeloid and B-lymphoid cells. Blood. 1993;82:2396-405. (Neut)