| Specificity |
Reacts with the Fc region of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3
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| Source |
Pooled antisera from goats hyperimmunized with mouse IgG
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| Cross Adsorption |
Mouse IgG Fab, IgM, and IgA; human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
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| Purification |
Affinity chromatography on mouse IgG covalently linked to agarose
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| Isotype |
Goat IgG
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| Isotype Control |
Goat IgG-UNLB
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| Format/Conjugate |
UNLB (Unconjugated)
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| Buffer Formulation |
Borate buffered saline, pH 8.2
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| Concentration |
1.0 mg/mL
|
| Volume |
1.0 mL
|
| Storage & Handling |
2-8°C
Please refer to product specific SDS
|
| Applications |
Quality tested applications for relevant formats include - ELISA
FLISA
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| Recommended Dilutions |
Please refer to product specific Technical Bulletin
|
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ELISA plate was coated with purified mouse IgG Fc and IgG Fab and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG Fc, Human/Bovine/Horse SP ads-UNLB (SB Cat. No. 1013-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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ELISA plate was coated with purified mouse IgG Fc, IgG Fab, IgM, and IgA and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG Fc, Human/Bovine/Horse SP ads conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).
ELISA plate was coated with purified mouse IgG Fc and IgG Fab and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG Fc, Human/Bovine/Horse SP ads-UNLB (SB Cat. No. 1013-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
ELISA plate was coated with purified mouse IgG Fc, IgG Fab, IgM, and IgA and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG Fc, Human/Bovine/Horse SP ads conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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