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Goat Anti-Mouse IgM, Human ads-TXRD

Cat. No.
1020-07  
Size
1.0 mg  
Price (USD)
$116.00 
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Specificity Reacts with the heavy chain of mouse IgM
Source Pooled antisera from goats hyperimmunized with mouse IgM
Cross Adsorption Mouse IgG and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgM covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-TXRD
Format/Conjugate TXRD (Texas Red®)
Buffer Formulation Supplied in PBS/NaN3
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-11
FLISA
Flow Cytometry 4,7,10,13-17
Other referenced applications for relevant formats include -
ELISpot 2,3,5,6,8-10,12
Immunohistochemistry-Frozen Sections 1,7,18-22
Immunohistochemistry-Paraffin Sections 28
Immunocytochemistry 1,13,23
Electron Microscopy 19
Immunoblotting 14,16,24
Immunoprecipitation 24
Purification 26
Stimulation 14,25-27
Recommended Dilutions Please refer to product specific Technical Bulletin
  • FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-TXRD (SB Cat. No. 1020-07).
  • Frozen mouse spleen tissue was stained with Goat Anti-Mouse IgM, Human ads-TXRD (SB Cat. No. 1020-07) and Rat Anti-Mouse IgD-BIOT (SB Cat. No. 1120-08) followed by Streptavidin-AF488.

    Image from Ren W, Lagerstedt K, Grimsholm O, Stern A, Sun J, Fang Y, et al. Uncoupling of natural IgE production and CD23 surface expression levels. PLoS One. 2013;8(4):e62851. Figure 2(c)
  • Frozen mouse lymph node section was stained with Goat Anti-Mouse IgM, Human ads-TXRD (SB Cat. No. 1020-07) followed by DAPI.

FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-TXRD (SB Cat. No. 1020-07).
 

Frozen mouse spleen tissue was stained with Goat Anti-Mouse IgM, Human ads-TXRD (SB Cat. No. 1020-07) and Rat Anti-Mouse IgD-BIOT (SB Cat. No. 1120-08) followed by Streptavidin-AF488.

Image from Ren W, Lagerstedt K, Grimsholm O, Stern A, Sun J, Fang Y, et al. Uncoupling of natural IgE production and CD23 surface expression levels. PLoS One. 2013;8(4):e62851. Figure 2(c)
 

Frozen mouse lymph node section was stained with Goat Anti-Mouse IgM, Human ads-TXRD (SB Cat. No. 1020-07) followed by DAPI.
 
References
1. Oliver AM, Martin F, Kearney JF. IgMhighCD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells. J Immunol. 1999;162:7198-207. (ELISA, IHC-FS, ICC)
2. Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon-γ production. Int J Exp Path. 2002;83:121-32. (ELISA, ELISPOT)
3. Hovden A, Cox RJ, Haaheim LR. Whole influenza virus vaccine is more immunogenic than split influenza virus vaccine and induces primarily an IgG2a response in BALB/c mice. Scand J Immunol. 2005;62:36-44. (ELISA, ELISPOT)
4. Ippolito GC, Schelonka RL, Zemlin M, Ivanov II, Kobayashi R, Zemlin C, et al. Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production. J Exp Med. 2006;203:1567-78. (ELISA, FC)
5. Huang L, Adachi T, Shimizu Y, Goto Y, Toyama J, Tanaka H, et al. Characterization of lectin isolated from Momordica charantia seed as a B cell activator. Immunol Lett. 2008;121:148-56. (ELISA, ELISPOT)
6. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Igα serine/threonines fine-tune Ig α tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA, ELISPOT)
7. Wang JH, Wu Q, Yang P, Li H, Li J, Mountz JD, et al. Type I IFN-dependent CD86high marginal zone precursor B cells are potent T cell costimulators. Arthritis Rheum. 2011;63:1054-64. (ELISA, FC, IHC-FS)
8. Conway KL, Kuballa P, Khor B, Zhang M, Shi HN, Virgin HW, et al. ATG5 regulates plasma cell differentiation. Autophagy. 2013;9:528-37. (ELISA, ELISPOT)
9. Goenka R, Matthews AH, Zhang B, O'Neill PJ, Scholz JL, Migone T, et al. Local BLyS production by T follicular cells mediates retention of high affinity B cells during affinity maturation. J Exp Med. 2014;211:45-56. (ELISA, ELISPOT)
10. Shen P, Roch T, Lampropoulou V, O'Connor RA, Stervbo U, Hilgenberg E, et al. IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases. Nature. 2014;507:366-70. (ELISA, ELISPOT, FC)
11. Pucella JN, Yen W, Kim MV, van der Veeken J, Socci ND, Naito Y, et al. miR-182 is largely dispensable for adaptive immunity: lack of correlation between expression and function. J Immunol. 2015;194:2635-42. (ELISA)
12. Shin C, Han J, Koh H, Choi B, Cho Y, Jeong H, et al. CD8α- dendritic cells induce antigen-specific T follicular helper cells generating efficient humoral immune responses. Cell Rep. 2015;11:1929-40. (ELISPOT)
13. Eaton-Bassiri AS, Mandik-Nayak L, Seo S, Madaio MP, Cancro MP, Erikson J. Alterations in splenic architecture and the localization of anti-double-stranded DNA B cells in aged mice. Int Immunol. 2000;12:915-26. (FC, ICC)
14. Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck H, Wabl M, et al. Ig heavy chain promotes mature B cell survival in the absence of light chain. J Immunol. 2007;179:1659-68. (FC, WB, Stim)
15. Zhou X, Xiang Y, Ding X, Garrard WT. A new hypersensitive site, HS10, and the enhancers, E3' and Ed, differentially regulate Igκ gene expression. J Immunol. 2012;188:2722-32. (FC)
16. Alkhatib A, Werner M, Hug E, Herzog S, Eschbach C, Faraidun H, et al. FoxO1 induces Ikaros splicing to promote immunoglobulin gene recombination. J Exp Med. 2012;209:395-406. (FC, WB)
17. Bai L, Deng S, Reboulet R, Mathew R, Teyton L, Savage PB, et al. Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides. Proc Natl Acad Sci USA. 2013;110:16097-102. (FC)
18. Raats CJ, van den Born J, Bakker MA, Oppers-Walgreen B, Pisa BJ, Dijkman HB, et al. Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies. Am J Pathol. 2000;156:1749-65. (IHC-FS, EM)
19. Mattsson N, Duzevik EG, Pelsue SC. Expansion of CD22lo B cells in the spleen of autoimmune-prone flaky skin mice. Cell Immunol. 2005;234:124-32. (IHC-FS)
20. Neese W, Westra J, van der Wal JE, Abbas F, Nicholas AP, Vissink A, et al. The periodontium of periodontitis patients contains citrullinated proteins which may play a role in ACPA (anti-citrullinated protein antibody) formation. J Clin Periodontol. 2012;39:599-607. (IHC-FS)
21. Simonetti G, Carette A, Silva K, Wang H, De Silva NS, Heise N, et al. IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity. J Exp Med. 2013;210:2887-902. (IHC-FS)
22. Ren W, Lagerstedt K, Grimsholm O, Stern A, Sun J, Fang Y, et al. Uncoupling of natural IgE production and CD23 surface expression levels. PLoS One. 2013;8(4):e62851. (IHC-FS)
23. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)
24. Elkabetz Y, Shapira I, Rabinovich E, Bar-Nun S. Distinct steps in dislocation of luminal endoplasmic reticulum-associated degradation substrates: roles of endoplamic reticulum-bound p97/Cdc48p and proteasome. J Biol Chem. 2004;279:3980-89. (WB, IP)
25. Kato I, Takai T, Kudo A. The pre-B cell receptor signaling for apoptosis is negatively regulated by FcγRIIB. J Immunol. 2002;168:629-34. (Stim)
26. Su Y, Flemming A, Wossning T, Hobeika E, Reth M, Jumaa H. Identification of a pre-BCR lacking surrogate light chain. J Exp Med. 2003;198:1699-706. (Purification, Stim)
27. Wolkerstorfer S, Schwaiger E, Rinnerthaler M, Gratz IK, Zoegg T, Brandstetter H, et al. HAX1 deletion impairs BCR internalization and leads to delayed BCR-mediated apoptosis. Cell Mol Immunol. 2015;Apr 13:doi: 10.1038/cmi.2015.018. [Epub ahead of print]. (Stim)
28. SouthernBiotech published data (IHC-PS)
Texas Red® is a registered trademark of Molecular Probes, Inc.
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