The Antibody Company - SouthernBiotech
 
   

Goat Anti-Mouse IgM-HRP

Cat. No.
1021-05  
Size
1.0 mL  
Price (USD)
$110.00 
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Specificity Reacts with the heavy chain of mouse IgM
Source Pooled antisera from goats hyperimmunized with mouse IgM
Cross Adsorption Mouse IgG and IgA; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgM covalently linked to agarose
Isotype Goat IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution in 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-6
FLISA
Flow Cytometry 4,7-17
Other referenced applications for relevant formats include -
ELISpot 6
Immunohistochemistry-Frozen Sections 18,19
Immunohistochemistry-Paraffin Sections 20
Immunocytochemistry 21,22
Immunoblotting 23,24
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM-HRP (SB Cat. No. 1021-05).

ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM-HRP (SB Cat. No. 1021-05).
 
References
1. Gass JN, Gifford NM, Brewer JW. Activation of an unfolded protein response during differentiation of antibody-secreting B cells. J Biol Chem. 2002;277:49047-54. (ELISA)
2. Chang P, Barral P, Fitch J, Pratama A, Ma CS, Kallies A, et al. Identification of Bcl-6-dependent follicular helper NKT cells that provide cognate help for B cell responses. Nat Immunol. 2012;13:35-43. (ELISA)
3. Sun Y, Peng I, Senger K, Hamidzadeh K, Reichelt M, Baca M, et al. Critical role of activation induced cytidine deaminase in experimental autoimmune encephalomyelitis. Autoimmunity. 2013;46:157-67. (ELISA)
4. Teague H, Harris M, Fenton J, Lallemand P, Shewchuk BM, Shaikh SR. Eicosapentaenoic and docosahexaenoic acid ethyl esters differentially enhance B-cell activity in murine obesity. J Lipid Res. 2014;55:1420-33. (ELISA, FC)
5. Merlo LM, Pigott E, DuHadaway JB, Grabler S, Metz R, Prendergast GC, et al. IDO2 is a critical mediator of autoantibody production and inflammatory pathogenesis in a mouse model of autoimmune arthritis. J Immunol. 2014;192:2082-90. (ELISA)
6. Patel PS, Kearney JF. Neonatal exposure to pneumococcal phosphorylcholine modulates the development of house dust mite allergy during adult life. J Immunol. 2015;194:5838-50. (ELISA, ELISPOT)
7. Chen C, Yull FE, Cardwell N, Singh N, Strayhorn WD, Nanney LB, et al. RAG2-/-, IκB-α-/- chimeras display a psoriasiform skin disease. J Invest Dermatol. 2000;115:1124-33. (FC)
8. Chen C, Singh N, Yull FE, Strayhorn D, Van Kaer L, Kerr LD. Lymphocytes lacking IκB-α develop normally, but have selective defects in proliferation and function. J Immunol. 2000;165:5418-27. (FC)
9. Erlandsson MC, Jonsson CA, Lindberg MK, Ohlsson C, Carlsten H. Raloxifene- and estradiol-mediated effects on uterus, bone and B lymphocytes in mice. J Endocrinol. 2002;175:319-27. (FC)
10. Islander U, Erlandsson MC, Hasséus B, Jonsson CA, Ohlsson C, Gustafsson J, et al. Influence of oestrogen receptor α and β on the immune system in aged female mice. Immunology. 2003;110:149-57. (FC)
11. Erlandsson MC, Jonsson CA, Islander U, Ohlsson C, Carlsten H. Oestrogen receptor specificity in oestradiol-mediated effects on B lymphopoiesis and immunoglobulin production in male mice. Immunology. 2003;108:346-51. (FC)
12. Bahjat FR, Pine PR, Reitsma A, Cassafer G, Baluom M, Grillo S, et al. An orally bioavailable spleen tyrosine kinase inhibitor delays disease progression and prolongs survival in murine lupus. Arthritis Rheum. 2008;58:1433-44. (FC)
13. Windahl SH, Andersson N, Chagin AS, Mårtensson UE, Carlsten H, Olde B, et al. The role of the G protein-coupled receptor GPR30 in the effects of estrogen in ovariectomized mice. Am J Physiol Endocrinol Metab. 2009;296:E490-6. (FC)
14. Giuriato S, Foisseau M, Dejean E, Felsher DW, Al Saati T, Demur C, et al. Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia. Blood. 2010;115:4061-70. (FC)
15. Verma-Gaur J, Hauser J, Grundström T. Negative feedback regulation of antigen receptors through calmodulin inhibition of E2A. J Immunol. 2012;188:6175-83. (FC)
16. Chen J, Yin H, Xu J, Wang Q, Edelblum KL, Sciammas R, et al. Reversing endogenous alloreactive B cell GC responses with anti-CD154 or CTLA-4Ig. Am J Transplant. 2013;13:2280-92. (FC)
17. Brady AM, Calix JJ, Yu J, Geno KA, Cutter GR, Nahm MH. Low invasiveness of pneumococcal serotype 11A is linked to ficolin-2 recognition of O-acetylated capsule epitopes and lectin complement pathway activation. J Infect Dis. 2014;210:1155-65. (FC)
18. DeAngelis RA, Markiewski MM, Kourtzelis I, Rafail S, Syriga M, Sandor A, et al. A complement-IL-4 regulatory circuit controls liver regeneration. J Immunol. 2012;188:641-8. (IHC-FS)
19. Dubois V, Laurent MR, Sinnesael M, Cielen N, Helsen C, Clinckemalie L, et al. A satellite cell-specific knockout of the androgen receptor reveals myostatin as a direct androgen target in skeletal muscle. FASEB J. 2014;28:2979-94. (IHC-FS)
20. Pamuk ON, Lapchak PH, Rani P, Pine P, Dalle Lucca JJ, Tsokos GC. Spleen tyrosine kinase inhibition prevents tissue damage after ischemia-reperfusion. Am J Physiol Gastrointest Liver Physiol. 2010;299:G391-9. (IHC-PS)
21. Gills JJ, Zhang C, Abu-Asab MS, Castillo SS, Marceau C, LoPiccolo J, et al. Ceramide mediates nanovesicle shedding and cell death in response to phosphatidylinositol ether lipid analogs and perifosine. Cell Death Differ. 2012;3:e340. (ICC)
22. Lilja HE, Morrison WA, Han X, Palmer J, Taylor C, Tee R, et al. An adipoinductive role of inflammation in adipose tissue engineering: key factors in the early development of engineered soft tissues. Stem Cells Dev. 2013;22:1602-13. (ICC)
23. Bergrin M, Bicer S, Lucas CA, Reiser PJ. Three-dimensional compartmentalization of myosin heavy chain and myosin light chain isoforms in dog thyroarytenoid muscle. Am J Physiol Cell Physiol. 2006;290:C1446-58. (WB)
24. Kuge H, Akahori K, Yagyu K, Honke K. Functional compartmentalization of the plasma membrane of neurons by a unique acyl chain composition of phospholipids. J Biol Chem. 2014;289:26783-93. (WB)
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