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Rat Anti-Mouse IL-3-LE/AF

Cat. No.
10212-14  
Size
0.5 mg  
Price (USD)
$300.00 
Qty
Add to Cart
Clone MP2-8F8
Isotype Rat IgG1κ
Isotype Control Mouse IgG1-LE/AF
Immunogen COS-expressed mouse IL-3
Specificity Mouse IL-3
Alternalte Name(s) Interleukin-3, burst promoting activity, BP/BPA, eosinophil colony stimulating factor, Eo-CSF, hematopoietic cell growth factor, HCGF, mast cell growth factor, MGF, MCGF, multi-colony stimulating factor, multi-CSF, P cell stimulating activity, PCSA
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Supplied in PBS
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at -20°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Capture – Quality tested 2-5
ELISpot-Capture – Reported in literature 6,7
Flow Cytometry – Reported in literature 8
Immunohistochemsitry-Frozen Sections – Reported in literature 9
Immunocytochemistry – Reported in literature 3
Immunoprecipitation – Reported in literature 1,2
Neutralization – Reported in literature 1-3
Multiplex-Capture – Reported in literature 4

Note – May be paired with the biotinylated clone MP2-43D11 (SB Cat. No. 10213-08) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Standard curve generated with Rat Anti-Mouse IL-3-UNLB (SB Cat. No. 10212-01; Clone MP2-8F8) and Rat Anti-Mouse IL-3-BIOT (SB Cat. No. 10213-08; Clone MP2-43D11) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Mouse IL-3-UNLB (SB Cat. No. 10212-01; Clone MP2-8F8) and Rat Anti-Mouse IL-3-BIOT (SB Cat. No. 10213-08; Clone MP2-43D11) followed by Streptavidin-HRP (SB Cat. No. 7100-05)
 
References
1. Abrams JS, Pearce MK. Development of rat anti-mouse interleukin 3 monoclonal antibodies which neutralize bioactivity in vitro. J Immunol. 1988;140:131-7. (Immunogen, IP, Neut)
2. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, IP, Neut)
3. Sander B, Höidén I, Andersson U, Möller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993;166:201-14. (ELISA-Capture, ICC, Neut)
4. Carson RT, Vignali DA. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999;227:41-52. (ELISA-Capture, Multiplex-Capture)
5. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
6. Karulin AY, Hesse MD, Tary-Lehmann M, Lehmann PV. Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity. J Immunol. 2000;164:1862-72. (ELISPOT-Capture)
7. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
8. Kim S, Prout M, Ramshaw H, Lopez AF, LeGros G, Min B. Cutting edge: basophils are transiently recruited into the draining lymph nodes during helminth infection via IL-3, but infection-induced Th2 immunity can develop without basophil lymph node recruitment or IL-3. J Immunol. 2010;184:1143-7. (FC)
9. Martinelli TM, Van Driel IR, Alderuccio F, Gleeson PA, Toh B. Analysis of mononuclear cell infiltrate and cytokine production in murine autoimmune gastritis. Gastroenterology. 1996;110:1791-802. (IHC-FS)
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