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Rat Anti-Mouse IL-17A-UNLB

Cat. No.
10214-01  
Size
0.5 mg  
Price (USD)
$255.00 
Qty
Add to Cart
Clone TC11-8H4
Isotype Rat IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen E. coli-expressed mouse IL-17
Specificity Mouse IL-17A
Alternalte Name(s) Interleukin-17A, cytotoxic T lymphocyte-associated antigen 8, CTLA-8
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Detection – Quality tested 1
Flow Cytometry – Quality tested 5
ELISpot-Capture – Reported in literature 2
ELISpot-Detection – Reported in literature 3,4

Note – May be paired with the purified clone TC11-18H10 (SB Cat. No. 10215-01) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
  • PMA and Ionomycin-stimulated C57BL/6N mouse T lymphocyte EL-4 cell line was stained with Rat Anti-Mouse IL-17A-FITC (SB Cat. No. 10214-02).

PMA and Ionomycin-stimulated C57BL/6N mouse T lymphocyte EL-4 cell line was stained with Rat Anti-Mouse IL-17A-FITC (SB Cat. No. 10214-02).
 
References
1. Amsen D, de Visser KE, Town T. Approaches to determine expression of inflammatory cytokines. Methods Mol Biol. 2009;511:107-42. (ELISA-Detection)
2. Kish DD, Li X, Fairchild RL. CD8 T cells producing IL-17 and IFN-γ initiate the innate immune response required for responses to antigen skin challenge. J Immunol. 2009;182:5949-59. (ELISPOT-Capture)
3. Faust SM, Lu G, Marini BL, Zou W, Gordon D, Iwakura Y, et al. Role of T cell TGFβ signaling and IL-17 in allograft acceptance and fibrosis associated with chronic rejection. J Immunol. 2009;183:7297-306. (ELISPOT-Detection)
4. Nekrasova T, Shive C, Gao Y, Kawamura K, Guardia R, Landreth G, et al. ERK1-deficient mice show normal T cell effector function and are highly susceptible to experimental autoimmune encephalomyelitis. J Immunol. 2005;175:2374-80. (ELISPOT-Detection)
5. Bending D, Newland S, Krejcí A, Phillips JM, Bray S, Cooke A. Epigenetic changes at Il12rb2 and Tbx21 in relation to plasticity behavior of Th17 cells. J Immunol. 2011;186:3373-82. (FC)
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