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Goat Anti-Mouse IgG, Human ads-TXRD

Cat. No.
1030-07  
Size
1.0 mg  
Price (USD)
$105.00 
Qty

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Specificity Reacts with the heavy chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Mouse IgM and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-TXRD
Format/Conjugate TXRD (Texas Red®)
Buffer Formulation Supplied in PBS/NaN3
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-10
FLISA
Flow Cytometry 5,11-13
Other referenced applications for relevant formats include -
ELISpot 1,3-6
Immunohistochemistry-Frozen Sections 2,14,15
Immunohistochemistry-Paraffin Sections 16-18
Immunocytochemistry 19-22
Immunoblotting 7,21,23-25
Multiplex 26
Recommended Dilutions Please refer to product specific Technical Bulletin
  • FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-TXRD (SB Cat. No. 1030-07).
  • Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Goat Anti-Mouse IgG, Human ads-TXRD (SB Cat. No. 1030-07) and DAPI.

FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-TXRD (SB Cat. No. 1030-07).
 

Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Goat Anti-Mouse IgG, Human ads-TXRD (SB Cat. No. 1030-07) and DAPI.
 
References
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2. Dunn LA, Upcroft JA, Fowler EV, Matthews BS, Upcroft P. Orally administered Giardia duodenalis extracts enhance an antigen-specific antibody response. Infect Immun. 2001;69:6503-10. (ELISA, IHC-FS)
3. Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon-γ production. Int J Exp Path. 2002;83:121-32. (ELISA, ELISPOT)
4. Hovden A, Cox RJ, Haaheim LR. Whole influenza virus vaccine is more immunogenic than split influenza virus vaccine and induces primarily an IgG2a response in BALB/c mice. Scand J Immunol. 2005;62:36-44. (ELISA, ELISPOT)
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7. Yolken RH, Jones-Brando L, Dunigan DD, Kannan G, Dickerson F, Severance E, et al. Chlorovirus ATCV-1 is part of the human oropharyngeal virome and is associated with changes in cognitive functions in humans and mice. Proc Natl Acad Sci USA. 2014;111:16106-11. (ELISA, WB)
8. Zhou Y, Maharaj PD, Mallajosyula JK, McCormick AA, Kearney CM. In planta production of flock house virus transencapsidated RNA and its potential use as a vaccine. Mol Biotechnol. 2015;57:325-36. (ELISA)
9. Zhao H, Bauzon F, Bi E, Yu JJ, Fu H, Lu Z, et al. Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age. J Biol Chem. 2015;290:5797-809. (ELISA)
10. Schubert RD, Hu Y, Kumar G, Szeto S, Abraham P, Winderl J, et al. IFN-β treatment requires B cells for efficacy in neuroautoimmunity. J Immunol. 2015;194:2110-6. (ELISA)
11. Chen J, Yin H, Xu J, Wang Q, Edelblum KL, Sciammas R, et al. Reversing endogenous alloreactive B cell GC responses with anti-CD154 or CTLA-4Ig. Am J Transplant. 2013;13:2280-92. (FC)
12. Maxwell P, Melendez-Rodríguez F, Matchett KB, Aragones J, Ben-Califa N, Jaekel H, et al. Novel antibodies directed against the human erythropoietin receptor: creating a basis for clinical implementation. Br J Haematol. 2015;168:429-42. (FC)
13. Brady AM, Spencer BL, Falsey AR, Nahm MH. Blood collection tubes influence serum ficolin-1 and ficolin-2 levels. Clin Vaccine Immunol. 2014;21:51-5. (FC)
14. Csencsits K, Burrell BE, Lu G, Eichwald EJ, Stahl GL, Bishop DK. The classical complement pathway in transplantation: unanticipated protective effects of C1q and role in inductive antibody therapy. Am J Transplant. 2008;8:1622-30. (IHC-FS)
15. Sic H, Kraus H, Madl J, Flittner K, von Münchow AL, Pieper K, et al. Sphingosine-1-phosphate receptors control B-cell migration through signaling components associated with primary immunodeficiencies, chronic lymphocytic leukemia, and multiple sclerosis. J Allergy Clin Immunol. 2014;134:420-8. (IHC-FS)
16. Zheng P, Zhao YX, Zhang AD, Kang C, Chen HC, Jin ML. Pathologic analysis of the brain from Streptococcus suis type 2 experimentally infected pigs. Vet Pathol. 2009;46:531-5. (IHC-PS)
17. McPhee CG, Sproule TJ, Shin D, Bubier JA, Schott WH, Steinbuck MP, et al. MHC class I family proteins retard systemic lupus erythematosus autoimmunity and B cell lymphomagenesis. J Immunol. 2011;187:4695-704. (IHC-PS)
18. Vujasinovic T, Pribic J, Kanjer K, Milosevic NT, Tomasevic Z, Milovanovic Z, et al. Gray-level co-occurrence matrix texture analysis of breast tumor images in prognosis of distant metastasis risk. Microsc Microanal. 2015;21:646-54. (IHC-PS)
19. Sjöberg M, Garoff H. Interactions between the transmembrane segments of the alphavirus E1 and E2 proteins play a role in virus budding and fusion. J Virol. 2003;77:3441-50. (ICC)
20. Roberts MS, Woods AJ, Dale TC, van der Sluijs P, Norman JC. Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of αvβ3 and α5β1 integrins. Mol Cell Biol. 2004;24:1505-15. (ICC)
21. Ma Y, Yates J, Liang Y, Lemon SM, Yi M. NS3 helicase domains involved in infectious intracellular hepatitis C virus particle assembly. J Virol. 2008;82:7624-39. (ICC, WB)
22. Mia MM, Bank RA. Paracrine factors of human amniotic fluid-derived mesenchymal stem cells show strong anti-fibrotic properties by inhibiting myofibroblast differentiation and collagen synthesis. J Stem Cell Res Ther. 2015;5:5. (ICC)
23. Friedman J, Alam SM, Shen X, Xia S, Stewart S, Anasti K, et al. Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. PLoS One. 2012;7(5):e37648. (WB)
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