Goat Anti-Mouse Lambda-Alexa Fluor® 555

Cat. No.
1.0 mg  
Price (USD)
Specificity Reacts with mouse λ light chains
Source Pooled antisera from goats hyperimmunized with mouse λ light chains
Cross Adsorption Mouse κ light chains; may react with λ light chains from other species
Purification Affinity chromatography on mouse λ light chains covalently linked to agarose
Isotype Goat IgG
Format/Conjugate AF555 (Alexa Fluor® 555)
Buffer Formulation Supplied in PBS/NaN3
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Quality tested applications for relevant formats include -
ELISA 1-6,23
Flow Cytometry 3,5,7-13,24
Other referenced applications for relevant formats include -
ELISpot 6,7
Immunohistochemistry-Frozen Sections 2,13-16
Immunohistochemistry-Paraffin Sections 17,23
Immunocytochemistry 1,4,18
Western Blot 19,20,25
Immunoprecipitation 21,22
Stimulation 23,24
Separation 25
Recommended Dilutions Please refer to product specific Technical Bulletin
  • FLISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Lambda-AF555 (SB Cat. No. 1060-32).
  • Frozen mouse lymph node section was stained with Goat Anti-Mouse Lambda-AF555 (SB Cat. No. 1060-32) followed by DAPI.

FLISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Lambda-AF555 (SB Cat. No. 1060-32).

Frozen mouse lymph node section was stained with Goat Anti-Mouse Lambda-AF555 (SB Cat. No. 1060-32) followed by DAPI.
1. Gollahon KA, Hagman J, Brinster RL, Storb U. Ig λ-producing B cells do not show feedback inhibition of gene rearrangement. J Immunol. 1988;141:2771-80. (ELISA, ICC)
2. Mandik-Nayak L, Nayak S, Sokol C, Eaton-Bassiri A, Madaio MP, Caton AJ, et al. The origin of anti-nuclear antibodies in bcl-2 transgenic mice. Int Immunol. 2000;12:353-64. (ELISA, IHC-FS)
3. Shivtiel S, Leider N, Melamed D. Receptor editing in CD45-deficient immature B cells. Eur J Immunol. 2002;32:2264-73. (ELISA, FC)
4. Ghumra A, Semblat J, McIntosh RS, Raza A, Rasmussen IB, Braathen R, et al. Identification of residues in the Cμ4 domain of polymeric IgM essential for interaction with Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1). J Immunol. 2008;181:1988-2000. (ELISA, ICC)
5. Liu X, Shen S, Manser T. Influence of B cell antigen receptor expression level on pathways of B cell tolerance induction. J Immunol. 2009;182:398-407. (ELISA, FC)
6. Willems KN. Regulation of humoral immunity by Pim kinases [dissertation]. Worcester (USA): University of Massachusetts; 2011. (ELISA, ELISPOT)
7. Seagal J, Edry E, Keren Z, Leider N, Benny O, Machluf M, et al. A fail-safe mechanism for negative selection of isotype-switched B cell precursors is regulated by the Fas/FasL pathway. J Exp Med. 2003;198:1609-19. (ELISPOT, FC)
8. Adachi T, Wakabayashi C, Nakayama T, Yakura H, Tsubata T. CD72 negatively regulates signaling through the antigen receptor of B cells. J Immunol. 2000;164:1223-9. (FC)
9. Su Y, Flemming A, Wossning T, Hobeika E, Reth M, Jumaa H. Identification of a pre-BCR lacking surrogate light chain. J Exp Med. 2003;198:1699-706. (FC)
10. Hayashi K, Nojima T, Goitsuka R, Kitamura D. Impaired receptor editing in the primary B cell repertoire of BASH-deficient mice. J Immunol. 2004;173:5980-8. (FC)
11. Vettermann C, Herrmann K, Albert C, Roth E, Bösl MR, Jäck H. A unique role for the λ5 nonimmunoglobulin tail in early B lymphocyte development. J Immunol. 2008;181:3232-42. (FC)
12. Heizmann B, Kastner P, Chan S. Ikaros is absolutely required for pre-B cell differentiation by attenuating IL-7 signals. J Exp Med. 2013;210:2823-32. (FC)
13. Aslam M, Kishi Y, Tsubata T. Excess CD40L does not rescue anti-DNA B cells from clonal anergy. F1000Res. 2013;2:218. (FC, IHC-FS)
14. Fukuda T, Yoshida T, Okada S, Hatano M, Miki T, Ishibashi K, et al. Disruption of the Bcl6 gene results in an impaired germinal center formation. J Exp Med. 1997;186:439-48. (IHC-FS)
15. Mandik-Nayak L, Huang G, Sheehan KC, Erikson J, Chaplin DD. Signaling through TNF receptor p55 in TNF-α-deficient mice alters the CXCL13/CCL19/CCL21 ratio in the spleen and induces maturation and migration of anergic B cells into the B cell follicle. J Immunol. 2001;167:1920-8. (IHC-FS, ICC)
16. Rouaud P, Saintamand A, Saad F, Carrion C, Lecardeur S, Cogné M, et al. Elucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region. J Exp Med. 2014;211:975-85. (IHC-FS)
17. Weinstein JS, Nacionales DC, Lee PY, Kelly-Scumpia KM, Yan X, Scumpia PO, et al. Colocalization of antigen-specific B and T cells within ectopic lymphoid tissue following immunization with exogenous antigen. J Immunol. 2008;181:3259-67. (IHC-PS)
18. Ghumra A, Shi J, Mcintosh RS, Rasmussen IB, Braathen R, Johansen F, et al. Structural requirements for the interaction of human IgM and IgA with the human Fcα/μ receptor. Eur J Immunol. 2009;39:1147-56. (ICC)
19. Elkabetz Y, Argon Y, Bar-Nun S. Cysteines in CH1 underlie retention of unassembled Ig heavy chains. J Biol Chem. 2005;280:14402-12. (WB)
20. Sato M, Adachi T, Tsubata T. Augmentation of signaling through BCR containing IgE but not that containing IgA due to lack of CD22-mediated signal regulation. J Immunol. 2007;178:2901-7. (WB)
21. Shen Y, Meunier L, Hendershot LM. Identification and characterization of a novel endoplasmic reticulum (ER) DnaJ homologue, which stimulates ATPase activity of BiP in vitro and is induced by ER stress. J Biol Chem. 2002;277:15947-56. (IP)
22. Behnke J, Hendershot LM. The large Hsp70 Grp170 binds to unfolded protein substrates in vivo with a regulation distinct from conventional Hsp70s. J Biol Chem. 2014;289:2899-907. (IP)
23. Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck H, Wabl M, et al. Ig heavy chain promotes mature B cell survival in the absence of light chain. J Immunol. 2007;179:1659-68. (ELISA, IHC-FS, Stim)
24. Übelhart R, Bach MP, Eschbach C, Wossning T, Reth M, Jumaa H. N-linked glycosylation selectively regulates autonomous precursor BCR function. Nat Immunol. 2010;11:759-65. (FC, Stim)
25. Yurovsky VV, Kelsoe G. Pairing of VH gene families with the λ1 light chain: evidence for a non-stochastic association. Eur J Immunol. 1993;23:1975-9. (WB, Sep)
Alexa Fluor® is a registered trademark of Life Technologies Corporation