Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-HRP

Cat. No.
1093-05  
Size
1.0 mL  
Price (USD)
$115.00 
Specificity Reacts with the heavy chain of mouse IgG2b
Source Pooled antisera from goats hyperimmunized with mouse IgG2b
Cross Adsorption Mouse IgG1, IgG2a, IgG3, IgM, and IgA; human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG2b covalently linked to agarose
Isotype Goat IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Other referenced applications for relevant formats include -
Immunocytochemistry 1
Western Blot 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-HRP (SB Cat. No. 1093-05).
  • Lane 1 - Untreated Jurkat whole cell lysate
    Lane 2 - TSA-treated Jurkat whole cell lysate (400 nM, 12 hr)
    Cell lysates were resolved by electrophoresis, transferred to PVDF membrane, probed with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01), and visualized using Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-HRP (SB Cat. No. 1093-05) secondary antibody and chemiluminescent detection.

ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-HRP (SB Cat. No. 1093-05).
 

Lane 1 - Untreated Jurkat whole cell lysate
Lane 2 - TSA-treated Jurkat whole cell lysate (400 nM, 12 hr)
Cell lysates were resolved by electrophoresis, transferred to PVDF membrane, probed with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01), and visualized using Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-HRP (SB Cat. No. 1093-05) secondary antibody and chemiluminescent detection.
 
References
1. SouthernBiotech published data (ICC, WB)