Rat Anti-Mouse IgG1-Alexa Fluor® 488

Cat. No.
0.1 mg  
Price (USD)
Clone SB77e
Isotype Rat (Sprague Dawley) IgG2bκ
Isotype Control Rat IgG2b-Alexa Fluor® 488
Specificity Mouse IgG1 Fc
Minimal reactivity to human, goat, and rabbit IgG and human, rat, hamster, bovine, horse, goat , sheep, chicken, guinea pig, donkey, and rabbit serum
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-5
ELISA – Quality tested 6-23
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 24
Immunohistochemistry-Paraffin Sections – Reported in literature 25
Immunocytochemistry – Reported in literature 26
Western Blot – Reported in literature 27
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794642
Gene ID
16017 (Mouse)
Gene Symbol
Ighg1 (Mouse)
Gene Aliases IgG1; Igh-4; VH7183
Uniprot ID
P01868 (Mouse)
P01869 (Mouse)
Uniprot Name
  • FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG1-AF488 (SB Cat. No. 1144-30).
  • Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Rat Anti-Mouse IgG1-AF488 (SB Cat. No. 1144-30).

FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG1-AF488 (SB Cat. No. 1144-30).

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Rat Anti-Mouse IgG1-AF488 (SB Cat. No. 1144-30).
Related Products (24)
1. Gilbertie JM. Increases in cortisol due to weaning stress and the subsequent alterations to immune function in beef calves [thesis]. Blacksburg (USA): Virginia Polytechnic Institute and State University; 2010. (FC)
2. Publicover J, Goodsell A, Nishimura S, Vilarinho S, Wang Z, Avanesyan L, et al. IL-21 is pivotal in determining age-dependent effectiveness of immune responses in a mouse model of human hepatitis B. J Clin Invest. 2011;121:1154-62. (FC)
3. Lucas AS. Bovine Coccidiosis: dynamics of infection in grazing cattle and the potential role of stress and immunity [dissertation]. Blacksburg (USA): Virginia Polytechnic Institute and State University; 2011. (FC)
4. Publicover J, Gagger A, Nishimura S, Van Horn CM, Goodsell A, Muench MO, et al. Age-dependent hepatic lymphoid organization directs successful immunity to hepatitis B. J Clin Invest. 2013;123:3728-39. (FC)
5. Poźniak B, Pawlak A, Obmińska-Mrukowicz B. Flow cytometric assessment of P-glycoprotein and multidrug resistance-associated protein activity and expression in canine lymphoma. In Vivo. 2015;29:149-54. (FC)
6. Su B, Wang J, Zhao G, Wang XJ, Li J, Wang B. Sequential administration of cytokine genes to enhance cellular immune responses and CD4+ T memory cells during DNA vaccination. Hum Vaccin Immunother. 2012;11:1659-67. (ELISA)
7. Reynolds LA. The immune response and the intestinal microbiota in control of susceptibility to Heligmosomoides polygyrus [dissertation]. Edinburgh (Scotland, United Kingdom): University of Edinburgh; 2012. (ELISA)
8. Pichler U, Hauser M, Hofer H, Himly M, Hoflehner E, Steiner M, et al. Allergen hybrids - next generation vaccines for Fagales pollen immunotherapy. Clin Exp Allergy. 2014;44:438-49. (ELISA)
9. Wagemakers A, Mason LM, Oei A, de Wever B, van der Poll T, Bins AD, et al. Rapid outer-surface protein C DNA tattoo vaccination protects against Borrelia afzelii infection. Gene Ther. 2014;21:1051-7. (ELISA)
10. Chen M, Hong MJ, Sun H, Wang L, Shi X, Gilbert BE, et al. Essential role for autophagy in the maintenance of immunological memory against influenza infection. Nat Med. 2014;20:503-10. (ELISA)
11. Sousa LM, Carneiro MB, Resende ME, Martins LS, Dos Santos LM, Vaz LG, et al. Neutrophils have a protective role during early stages of Leishmania amazonensis infection in BALB/c mice. Parasite Immunol. 2014;36:13-31. (ELISA)
12. Joshi VB, Adamcakova-Dodd A, Jing X, Wongrakpanich A, Gibson-Corley KN, Thorne PS, et al. Development of a poly (lactic-co-glycolic acid) particle vaccine to protect against house dust mite induced allergy. AAPS J. 2014;16:975-85. (ELISA)
13. Patel JM, Vartabedian VF, Kim M, He S, Kang S, Selvaraj P. Influenza virus-like particles engineered by protein transfer with tumor-associated antigens induces protective antitumor immunity. Biotechnol Bioeng. 2015;112:1102-10. (ELISA)
14. Zuest R, Valdes I, Skibinski D, Lin Y, Toh YX, Chan K, et al. Tetravalent dengue DIIIC protein together with alum and ODN elicits a Th1 response and neutralizing antibodies in mice. Vaccine. 2015;33:1474-82. (ELISA)
15. Funakoshi S, Shimizu T, Numata O, Ato M, Melchers F, Ohnishi K. BILL-cadherin/cadherin-17 contributes to the survival of memory B cells. PLoS One. 2015;10(1):e0117566. (ELISA)
16. Chua BY, Sekiya T, Al Kobaisi M, Short KR, Mainwaring DE, Jackson DC. A single dose biodegradable vaccine depot that induces persistently high levels of antibody over a year. Biomaterials. 2015;53:50-7. (ELISA)
17. Patel JM, Kim M, Vartabedian VF, Lee Y, He S, Song J, et al. Protein transfer-mediated surface engineering to adjuvantate virus-like nanoparticles for enhanced anti-viral immune responses. Nanomedicine. 2015;11:1097-107. (ELISA)
18. Meli AP, Fontés G, Avery DT, Leddon SA, Tam M, Elliot M, et al. The integrin LFA-1 controls T follicular helper cell generation and maintenance. Immunity. 2016;45:831-46. (ELISA)
19. Fu L, Peng J, Zhao S, Zhang Y, Su X, Wang Y. Lactic acid bacteria-specific induction of CD4+Foxp3+T cells ameliorates shrimp tropomyosin-induced allergic response in mice via suppression of mTOR signaling. Sci Rep. 2017;7:1987. (ELISA)
20. Hirschl S, Ralser C, Asam C, Gangitano A, Huber S, Ebner C, et al. Expression and characterization of functional recombinant Bet v 1.0101 in the chloroplast of Chlamydomonas reinhardtii. Int Arch Allergy Immunol. 2017;173:44-50. (ELISA)
21. Yilmaz Ç, Özcengiz E, Özcengiz G. Recombinant outer membrane protein Q and putative lipoprotein from Bordetella pertussis inducing strong humoral response were not protective alone in the murine lung colonization model. Turk J Biol. 2018; 42:123-31. (ELISA)
22. Tong P, Granato A, Zuo T, Chaudhary N, Zuiani A, Han SS, et al. IgH isotype-specific B cell receptor expression influences B cell fate. Proc Natl Acad Sci USA. 2017;114:E8411-20. (ELISA)
23. Wolf M, Aglas L, Twaroch TE, Steiner M, Huber S, Hauser M, et al. Endolysosomal protease susceptibility of Amb a 1 as a determinant of allergenicity. J Allergy Clin Immunol. 2018;141:1488-91.e5. (ELISA)
24. Punt S, Houwing-Duistermaat JJ, Schulkens IA, Thijssen VL, Osse EM, de Kroon CD, et al. Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer. Mol Cancer. 2015;14:71. (IHC-FS)
25. Adepu S, Rosman CW, Dam W, van Dijk MC, Navis G, van Goor H, et al. Incipient renal transplant dysfunction associates with tubular syndecan-1 expression and shedding. Am J Physiol Renal Physiol. 2015;309:F137-45. (IHC-PS)
26. Jimenez R, Melo EO, Davydenko O, Ma J, Mainigi M, Franke V, et al. Maternal SIN3A regulates reprogramming of gene expression during mouse preimplantation development. Biol Reprod. 2015;93:89. (ICC)
27. Beauchamp JM, Erfurt RS, DiRita VJ. Characterization and localization of the Campylobacter jejuni transformation system proteins CtsE, CtsP, and CtsX. J Bacteriol. 2015;197:636-45. (WB)
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