Mouse Anti-Human MMP-9-UNLB

Cat. No.
12025-01  
Size
0.1 mg  
Price (USD)
$195.00 
Clone SB15c
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-UNLB
Immunogen Recombinant full length MMP-9
Specificity Human/Mouse MMP-9
Alternalte Name(s) Matrix metalloproteinase 9, gelatinase B, 92 kDa gelatinase
Description Matrix metalloproteinases (MMPs) are a family of at least 23 structurally related, zinc-containing enzymes that have the ability to breakdown connective tissue. MMP-9 has shown substrate specificity to collagens types IV, V, XI, and XIV as well as additional extracellular matrix components including gelatin, elastin, vitronectin, and laminin.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Immunohistochemistry-Paraffin Sections – Quality tested 1-3
ELISA 5
Immunocytochemistry 5
Western Blot – Reported in literature 4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794705
Gene ID
4318 (Human)
17395 (Mouse)
Gene Symbol
MMP9 (Human)
Mmp9 (Mouse)
Gene Aliases CLG4B; GELB; MANDP2; MMP-9 AW743869; B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9
Uniprot ID
P14780 (Human)
P41245 (Mouse)
Uniprot Name
  • Paraffin embedded human gastric cancer tissue was stained with Mouse IgG2a-UNLB isotype control (SB Cat. No. 0103-01; left) and Mouse Anti-Human MMP-9-UNLB (SB Cat. No. 12025-01; right) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05), DAB, and hematoxylin.
  • Paraffin embedded human colon cancer tissue was stained with Mouse Anti-Human MMP-9-UNLB (SB Cat. No. 12025-01) followed by Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.

Paraffin embedded human gastric cancer tissue was stained with Mouse IgG2a-UNLB isotype control (SB Cat. No. 0103-01; left) and Mouse Anti-Human MMP-9-UNLB (SB Cat. No. 12025-01; right) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05), DAB, and hematoxylin.
 

Paraffin embedded human colon cancer tissue was stained with Mouse Anti-Human MMP-9-UNLB (SB Cat. No. 12025-01) followed by Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
 
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References
1. Ye H, Yu T, Temam S, Ziober BL, Wang J, Schwartz JL, et al. Transcriptomic dissection of tongue squamous cell carcinoma. BMC Genomics. 2008;9:69. (IHC-PS)
2. Ouvrier A, Alves G, Damon-Soubeyrand C, Marceau G, Cadet R, Janny L, et al. Dietary cholesterol-induced post-testicular infertility. PLoS One. 2011;6(11):e26966. (IHC-PS, Mouse Reactivity)
3. Stenvold H, Donnem T, Andersen S, Al-Saad S, Al-Shibli K, Busund L, et al. Overexpression of matrix metalloproteinase-7 and -9 in NSCLC tumor and stromal cells: correlation with a favorable clinical outcome. Lung Cancer. 2012;75:235-41. (IHC-PS)
4. Liu G, Liu Y, Yang Z, Zhu A, Zhao C. MicroRNA-524-5p suppresses the growth and invasive abilities of gastric cancer cells. Oncol Lett. 2016;11:1926-32. (WB)
5. SouthernBiotech unpublished data (ELISA, ICC)