Rat Anti-Mouse CD106-LE/AF

Cat. No.
1510-14  
Size
0.5 mg   		Price (USD)
$240.00 
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Clone M/K-2
Isotype Rat (Fisher) IgG1κ
Isotype Control Rat IgG1-LE/AF
Immunogen BALB/3T3 and +/+2.4 cells
Specificity Mouse CD106
Alternalte Name(s) VCAM-1, INCAM-110
Description CD106, also known as VCAM-1, is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (e.g., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody MK-2 has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein. The M/K-2 monoclonal antibody immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under non-reducing conditions.
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; handle under aseptic conditions
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 4,8,9
Immunohistochemistry-Frozen Sections – Reported in literature 4-7
Immunoprecipitation – Reported in literature 1,2
Western Blot – Reported in literature 2
Adhesion – Reported in literature 1-3
Blocking – Reported in literature 1-3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794784
Gene ID
22329 (Mouse)
Gene Symbol
Vcam1 (Mouse)
Gene Aliases CD106; Vcam-1
Uniprot ID
P29533 (Mouse)
Uniprot Name
VCAM1_MOUSE 
  • Rat Anti-Mouse CD106-PE
    BALB/c mouse bone marrow cells were stained with Rat Anti-Mouse CD106-PE (SB Cat. 1510-09) and Rat Anti-Mouse Ly-6G/Ly-6C-FITC (SB Cat. No. 1900-02).

BALB/c mouse bone marrow cells were stained with Rat Anti-Mouse CD106-PE (SB Cat. 1510-09) and Rat Anti-Mouse Ly-6G/Ly-6C-FITC (SB Cat. No. 1900-02).
 
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References
1. Miyake K, Weissman IL, Greenberger JS, Kincade PW. Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. J Exp Med. 1991;173:599-607. (Immunogen, IP, Block, Adhesion)
2. Li W, Ishihara K, Yokota T, Nakagawa T, Koyama N, Jin J, et al. Reduced α4β1 integrin/VCAM-1 interactions lead to impaired pre-B cell repopulation in alpha 1,6-fucosyltransferase deficient mice. Glycobiology. 2008;18:114-24. (IP, WB, Block, Adhesion)
3. Miyake K, Medina K, Ishihara K, Kimoto M, Auerbach R, Kincade PW. A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture. J Cell Biol. 1991;114:557-65. (Block, Adhesion)
4. Ulyanova T, Scott LM, Priestley GV, Jiang Y, Nakamoto B, Koni PA, et al. VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin. Blood. 2005;106:86-94. (IHC-FS, FC)
5. Hechler B, Freund M, Ravanat C, Magnenat S, Cazenave J, Gachet C. Reduced atherosclerotic lesions in P2Y1/apolipoprotein E double-knockout mice: the contribution of non-hematopoietic-derived P2Y1 receptors. Circulation. 2008;118:754-63. (IHC-FS)
6. Glanville SH, Bekiaris V, Jenkinson EJ, Lane PJ, Anderson G, Withers DR. Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization. Eur J Immunol. 2009;39:280-9. (IHC-FS)
7. Song J, Lokmic Z, Lämmermann T, Rolf J, Wu C, Zhang X, et al. Extracellular matrix of secondary lymphoid organs impacts on B-cell fate and survival. Proc Natl Acad Sci USA. 2013;110(31):E2915-24. (IHC-FS)
8. Ambardekar VV, Han H, Varney ML, Vinogradov SV, Singh RK, Vetro JA. The modification of siRNA with 3' cholesterol to increase nuclease protection and suppression of native mRNA by select siRNA polyplexes. Biomaterials. 2011;32:1404-11. (FC)
9. Banerjee ER, Henderson WR. Defining the molecular role of gp91phox in the immune manifestation of acute allergic asthma using a preclinical murine model. Clin Mol Allergy. 2012;10:2. (FC)