Hamster Anti-Mouse CD3ε-LE/AF

Cat. No.
1531-14  
Size
0.5 mg   		Price (USD)
$198.00 
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Clone 500-A2
Isotype Hamster (Syrian) IgG2
Isotype Control Hamster IgG-LE/AF
Immunogen Immune complexes isolated from C6VL-BS thymoma cells
Specificity Mouse CD3ε
Alternalte Name(s) CD3 epsilon
Description CD3ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3ε is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. 500-A2 recognizes an epitope on the 25 kDa ε chain of the CD3/TCR complex of mouse strains tested.
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; handle under aseptic conditions
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunoprecipitation – Reported in literature 1
Activation – Reported in literature 1,2,4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794815
Gene ID
12501 (Mouse)
Gene Symbol
Cd3e (Mouse)
Gene Aliases AI504783; CD3; CD3epsilon; T3e
Uniprot ID
P22646 (Mouse)
Uniprot Name
CD3E_MOUSE 
  • Hamster Anti-Mouse CD3e-APC
    BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD3ε-APC (SB Cat. No. 1531-11) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).

BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD3ε-APC (SB Cat. No. 1531-11) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
 
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References
1. Allison JP, Havran WL, Poenie M, Kimura J, DeGraffenreid L, Ajami M, et al. Expression and function of CD3 on murine thymocytes. In: Davis MM, Kappler J, editors. The T-Cell Receptor, UCLA Symposia, 73rd Edition. New York: Alan R. Liss; 1988. p.33-45. (Immunogen, FC, IP, Activ)
2. Swallow MM, Wallin JJ, Sha WC. B7h, a novel costimulatory homolog of B7.1 and B7.2, is induced by TNFα. Immunity. 1999;11:423-32. (Activ)
3. Hörnquist CE, Lu X, Rogers-Fani PM, Rudolph U, Shappell S, Birnbaumer L, et al. Gαi2-deficient mice with colitis exhibit a local increase in memory CD4+ T cells and proinflammatory Th1-type cytokines. J Immunol. 1997;158:1068-77. (IHC-FS)
4. Portoles P, Rojo J, Golby A, Bonneville M, Gromkowski S, Greenbaum L, et al. Monoclonal antibodies to murine CD3ε define distinct epitopes one of which may interact with CD4 during T cell activation. J Immunol. 1989;142:4169-75. (Activ)