Rat Anti-Mouse CD11a-LE/AF

Cat. No.
1555-14  
Size
0.5 mg  
Price (USD)
$255.00 
Clone I21/7
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-LE/AF
Immunogen Whole BW5147 cells
Specificity Mouse/Human CD11a
Alternalte Name(s) LFA-1, LFA-1α, integrin αL
Description The monoclonal antibody I21/7 reacts with the α subunit of mouse and human CD11a, also known as LFA-1, and a member of the integrin family of cell adhesion molecules. The antigen is expressed on thymocytes, T and B lymphocytes, and on non-lymphoid cells in the bone marrow. CD11a appears to be an essential component for T-cell activation by antigen-presenting cells and is important for cell-mediated killing. Costimulatory signaling by LFA-1 is achieved by pairing with its ligand, CD54/ICAM-1.
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling -20°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 5-8
Immunohistochemistry-Frozen Sections – Reported in literature 2
Immunocytochemistry – Reported in literature 3
Immunoprecipitation – Reported in literature 1,4
Blocking – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794894
Gene ID
16408 (Mouse)
3683 (Human)
Gene Symbol
Itgal (Mouse)
ITGAL (Human)
Gene Aliases Cd11a; LFA-1; Ly-15; Ly-21; (p180); LFA-1A; CD11A; LFA1A
Uniprot ID
P24063 (Mouse)
P20701 (Human)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD11a-PE/CY7 (SB Cat. No. 1555-17).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD11a-PE/CY7 (SB Cat. No. 1555-17).
 
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References
1. Trowbridge IS, Omary MB. Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1. J Exp Med. 1981;154:1517-24. (Immunogen, IP, Block)
2. Pauschinger M, Noutsias M, Lassner D, Schultheiss HP, Kuehl U. Inflammation, ECG changes and pericardial effusion: whom to biopsy in suspected myocarditis?. Clin Res Cardiol. 2006;95:569-83. (IHC-FS, Human Reactivity)
3. Thauland TJ, Koguchi Y, Wetzel SA, Dustin ML, Parker DC. Th1 and Th2 cells form morphologically distinct immunological synapses. J Immunol. 2008;181:393-9. (ICC)
4. Lefrançois L, Bevan MJ. Novel antigenic determinants of the T200 glycoprotein expressed preferentially by activated cytotoxic T lymphocytes. J Immunol. 1984;135:374-83. (IP)
5. Kato N, Yuzawa Y, Kosugi T, Hobo A, Sato W, Miwa Y, et al. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. J Am Soc Nephrol. 2009;20:1565-76. (FC)
6. Takeshi T, Keisuke N, Takaaki I, Makoto Y, Tatsuji N. Involvement of adhesion molecule in in vitro plaque-like formation of macrophages stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide. J Periodont Res. 2010;45:550-6. (FC)
7. Nagaoka K, Takahara K, Minamino K, Takeda T, Yoshida Y, Inaba K. Expression of C-type lectin, SIGNR3, on subsets of dendritic cells, macrophages, and monocytes. J Leukoc Biol. 2010;88:913-24. (FC)
8. Domingos-Pereira S, Decrausaz L, Derré L, Bobst M, Romero P, Schiller JT, et al. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice. Mucosal Immunol. 2013;6:393-404. (FC)