Rat Anti-Mouse CD31-Alexa Fluor® 488

Cat. No.
1625-30  
Size
0.1 mg   		Price (USD)
$190.00 
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Clone 390
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-Alexa Fluor® 488
Immunogen C3H/HeJ mouse hematopoietic progenitor cell line 32D
Specificity Mouse CD31
Alternalte Name(s) Platelet endothelial cell adhesion molecule, PECAM-1, endoCAM, GPIIa
Description CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 interacts homotypically in cell adhesion assays. The monoclonal antibody 390 inhibits the aggregation of L cells transfected with a variant form of CD31.
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,10-12,14
Immunohistochemistry-Frozen Sections – Reported in literature 1-8
Immunocytochemistry – Reported in literature 9
Immunoprecipitation – Reported in literature 1,14
Blocking – Reported in literature 13,14
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795052
Gene ID
18613 (Mouse)
Gene Symbol
Pecam1 (Mouse)
Gene Aliases Cd31; Pecam; C85791; PECAM-1
Uniprot ID
Q08481 (Mouse)
Uniprot Name
PECA1_MOUSE 
  • Rat Anti-Mouse CD31-AF488
    BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD31-AF488 (SB Cat. No. 1625-30).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD31-AF488 (SB Cat. No. 1625-30).
 
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References
1. Baldwin HS, Shen HM, Yan H, DeLisser HM, Chung A, Mickanin C, et al. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development. 1994;120:2539-53. (Immunogen, FC, IP, IHC-FS)
2. Popa ER, Harmsen MC, Tio RA, van der Strate BW, Brouwer LA, Schipper M, et al. Circulating CD34+ progenitor cells modulate host angiogenesis and inflammation in vivo. J Mol Cell Cardiol. 2006;41:86-96. (IHC-FS)
3. Salmon BA, Siemann DW. Characterizing the tumor response to treatment with combretastatin A4 phosphate. Int J Radiat Oncol Biol Phys. 2007;68:211-7. (IHC-FS)
4. Xu Y, Arai H, Murayama T, Kita T, Yokode M. Hypercholesterolemia contributes to the development of atherosclerosis and vascular remodeling by recruiting bone marrow-derived cells in cuff-induced vascular injury. Biochem Biophys Res Commun. 2007;363:782-7. (IHC-FS)
5. Kerber M, Reiss Y, Wickersheim A, Jugold M, Kiessling F, Heil M, et al. Flt-1 signaling in macrophages promotes glioma growth in vivo. Cancer Res. 2008;68:7342-51. (IHC-FS)
6. Sarraf-Yazdi S, Mi J, Moeller BJ, Niu X, White RR, Kontos CD, et al. Inhibition of in vivo tumor angiogenesis and growth via systemic delivery of an angiopoietin 2-specific RNA aptamer. J Surg Res. 2008;146:16-23. (IHC-FS)
7. Kojima T, Kanemaru S, Hirano S, Tateya I, Ohno S, Nakamura T, et al. Regeneration of radiation damaged salivary glands with adipose-derived stromal cells. Laryngoscope. 2011;121:1864-9. (IHC-FS)
8. Villalta SA, Lang J, Kubeck S, Kabre B, Szot GL, Calderon B, et al. Inhibition of VEGFR-2 reverses type 1 diabetes in NOD mice by abrogating insulitis and restoring islet function. Diabetes. 2013;62:2870-8. (IHC-FS)
9. Zhang H, Nieves JL, Fraser ST, Isern J, Douvaras P, Papatsenko D, et al. Expression of podocalyxin separates the hematopoietic and vascular potentials of mouse embryonic stem cell-derived mesoderm. Stem Cells. 2014;32:191-203. (ICC)
10. Pevsner-Fischer M, Morad V, Cohen-Sfady M, Rousso-Noori L, Zanin-Zhorov A, Cohen S, et al. Toll-like receptors and their ligands control mesenchymal stem cell functions. Blood. 2007;109:1422-32. (FC)
11. Motohashi N, Uezumi A, Yada E, Fukada S, Fukushima K, Imaizumi K, et al. Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts. Am J Pathol. 2008;173:781-91. (FC)
12. Cao G, Fehrenbach ML, Williams JT, Finklestein JM, Zhu J, DeLisser HM. Angiogenesis in platelet endothelial cell adhesion molecule-1-null mice. Am J Pathol. 2009;175:903-15. (FC)
13. DeLisser HM, Christofidou-Solomidou M, Strieter RM, Burdick MD, Robinson CS, Wexler RS, et al. Involvement of endothelial PECAM-1/CD31 in angiogenesis. Am J Pathol. 1997;151:671-7. (Block)
14. Rosenblum WI, Nelson GH, Wormley B, Werner P, Wang J, Shih CC. Role of platelet-endothelial cell adhesion molecule (PECAM) in platelet adhesion/aggregation over injured but not denuded endothelium in vivo and ex vivo. Stroke. 1996;27:709-11. (Block, FC, IP)
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