Rat Anti-Mouse CD54-Alexa Fluor® 488

Cat. No.
1701-30  
Size
0.1 mg  
Price (USD)
$192.00 
Clone YN1/1.7.4
Isotype Rat (Fisher334) IgG2bκ
Isotype Control Rat IgG2b-Alexa Fluor® 488
Immunogen Mouse NS-1 cells
Specificity Mouse/Porcine CD54
Alternalte Name(s) ICAM-1, Ly-47
Description CD54, a member of the immunoglobulin superfamily of cell surface receptors and also known as Intercellular Adhesion Molecule-1 (ICAM-1), is a type I transmembrane glycoprotein that mediates important cell/cell interactions. CD54 is expressed on endothelial cells, dendritic cells, keratinocytes, and lymphocytes. Its expression is upregulated by inflammatory mediators such as IFN γ, IL-1, TNF-α, lipopolysaccharide, and phorbol esters. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels, particularly in areas of inflammation. CD54 on antigen-presenting cells contributes to antigen-specific T-cell activation, presumably by enhancing interactions between T cell and antigen-presenting cells. These adhesion reactions are mediated via the binding of CD54 to its major ligands, LFA-1 and Mac-1.
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 9-11
Immunohistochemistry-Frozen Sections – Reported in literature 3,12-14
Immunocytochemistry – Reported in literature 4
Western Blot – Reported in literature 6-8
Immunoprecipitation – Reported in literature 1
Blocking – Reported in literature 1,2
Purification – Reported in literature 5
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795159
Gene ID
15894 (Mouse)
396750 (Porcine)
Gene Symbol
Icam1 (Mouse)
ICAM1 (Porcine)
Gene Aliases CD54; Icam-1; Ly-47; MALA-2
Uniprot ID
P13597 (Mouse)
Uniprot Name
  • C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD54-AF488 (SB Cat. No. 1701-30) and Rat Anti-Mouse CD49b-PE (SB Cat. No. 1806-09).

C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD54-AF488 (SB Cat. No. 1701-30) and Rat Anti-Mouse CD49b-PE (SB Cat. No. 1806-09).
 
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References
1. Takei F. Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2). J Immunol. 1985;134:1403-7. (Immunogen, FC, IP, Block)
2. Kumasaka T, Quinlan WM, Doyle NA, Condon TP, Sligh J, Takei F, et al. Role of the intercellular adhesion molecule-1(ICAM-1) in endotoxin-induced pneumonia evaluated using ICAM-1 antisense oligonucleotides, anti-ICAM-1 monoclonal antibodies, and ICAM-1 mutant mice. J Clin Invest. 1996;97:2362-9. (Block)
3. Song J, Lokmic Z, Lämmermann T, Rolf J, Wu C, Zhang X, et al. Extracellular matrix of secondary lymphoid organs impacts on B-cell fate and survival. Proc Natl Acad Sci USA. 2013;110(31):E2915-24. (IHC-FS)
4. Bosch B, Heipertz EL, Drake JR, Roche PA. MHC class II-peptide complexes arrive at the plasma membrane in cholesterol-rich microclusters. J Biol Chem. 2013;288:13236-42. (ICC)
5. Krauss K, Altevogt P. Integrin leukocyte function-associated antigen-1-mediated cell binding can be activated by clustering of membrane rafts. J Biol Chem. 1999;274:36921-7. (Purification)
6. Muntasell A, Berger AC, Roche PA. T cell-induced secretion of MHC class II–peptide complexes on B cell exosomes. EMBO J. 2007;26:4263-72. (WB)
7. Ramachandra L, Qu Y, Wang Y, Lewis CJ, Cobb BA, Takatsu K, et al. Mycobacterium tuberculosis synergizes with ATP to induce release of microvesicles and exosomes containing major histocompatibility complex class II molecules capable of antigen presentation. Infect Immun. 2010;78:5116-25. (WB)
8. Chen L, Lin S, Amin S, Overbergh L, Maggiolino G, Chan LS. VCAM-1 blockade delays disease onset, reduces disease severity and inflammatory cells in an atopic dermatitis model. Immunol Cell Biol. 2010;88:334-42. (WB)
9. Zaru R, Mollahan P, Watts C. 3-Phosphoinositide-dependent kinase 1 deficiency perturbs toll-like receptor signaling events and actin cytoskeleton dynamics in dendritic cells. J Biol Chem. 2008;283:929-39. (FC)
10. Podgrabinska S, Kamalu O, Mayer L, Shimaoka M, Snoeck H, Randolph GJ, et al. Inflamed lymphatic endothelium suppresses dendritic cell maturation and function via Mac-1/ICAM-1-dependent mechanism. J Immunol. 2009;183:1767-79. (FC)
11. Jacobsen J, Haabeth OW, Tveita AA, Schjetne KW, Munthe LA, Bogen B. Naive idiotope-specific B and T cells collaborate efficiently in the absence of dendritic cells. J Immunol. 2014;192:4174-83. (FC)
12. Hoehn D, Sun L, Sucosky P. Role of pathologic shear stress alterations in aortic valve endothelial activation. Cardiovasc Eng Technol. 2010;1:165-78. (IHC-FS, Porcine Reactivity)
13. Sun L, Chandra S, Sucosky P. Ex vivo evidence for the contribution of hemodynamic shear stress abnormalities to the early pathogenesis of calcific bicuspid aortic valve disease. PLoS One. 2012;7(10):e48843. (IHC-FS, Porcine Reactivity)
14. Sun L, Rajamannan NM, Sucosky P. Defining the role of fluid shear stress in the expression of early signaling markers for calcific aortic valve disease. PLoS One. 2013;8(12):e84433. (IHC-FS, Porcine Reactivity)
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