Hamster Anti-Mouse CD69-Alexa Fluor® 488

Cat. No.
1715-30  
Size
0.1 mg   		Price (USD)
$199.00 
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Technical Bulletin Certificate of
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Clone H1.2F3
Isotype Hamster (Armenian) IgG1
Isotype Control Hamster IgG-Alexa Fluor® 488
Immunogen Mouse dendritic epidermal cell line Y245
Specificity Mouse CD69
Alternalte Name(s) VEA, very early activation antigen, AIM, EA1, MLR3, gp34/28, Leu-23
Description CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C-type lectin most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. Although not detectable on resting lymphocytes, its expression is rapidly upregulated upon activation of T, B and NK cells, and neutrophils. Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. The monoclonal antibody H1.2F3 augments PMA-induced T-cell proliferation and induces redirected lysis of Fc receptor-bearing target cells by NK cells.
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunoprecipitation – Reported in literature 1
Costimulation – Reported in literature 1
Activation – Reported in literature 2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795186
Gene ID
12515 (Mouse)
Gene Symbol
Cd69 (Mouse)
Gene Aliases AIM; VEA; AI452015; 5830438K24Rik
Uniprot ID
P37217 (Mouse)
Uniprot Name
CD69_MOUSE 
  • Hamster Anti-Mouse CD69-AF488
    PMA and ionomycin stimulated BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD69-AF488 (SB Cat. No. 1715-30).

PMA and ionomycin stimulated BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD69-AF488 (SB Cat. No. 1715-30).
 
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References
1. Yokoyama WM, Koning F, Kehn PJ, Pereira GM, Stingl G, Coligan JE, et al. Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation. J Immunol. 1988;141:369-76. (Immunogen, FC, IP, Costim)
2. Karlhofer FM, Yokoyama WM. Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways. J Immunol. 1991;146:3662-73. (FC, Activ)
3. Podd BS, Thoits J, Whitley N, Cheng H, Kudla KL, Taniguchi H, et al. T cells in cryptopatch aggregates share TCR γ variable region junctional sequences with γδ T cells in the small intestinal epithelium of mice. J Immunol. 2006;176:6532-42. (IHC-FS)
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