Rat Anti-Human Caspase-8-UNLB

Cat. No.
17300-01  
Size
0.1 mg  
Price (USD)
$185.00 
Clone SB125a
Isotype Rat (Sprague Dawley) IgMκ
Isotype Control Rat IgM-UNLB
Immunogen Purified native human caspase-8
Specificity Human caspase-8
Alternalte Name(s) MACH, FLICE, Mch5
Description The caspases are a family of cysteine proteases that induce apoptosis in response to a variety of intra- and extracellular stimuli. These enzymes are synthesized as inactive precursors which become cleaved at aspartate-specific sites upon induction of apoptosis. Caspase-8 is a cytosolic protease that is capable of processing/activating all known caspases, indicating its presence early in the apoptotic cascade. It mediates cell death induction by receptors of the tumor necrosis factor (TNF) and nerve growth factor (NGF) family to eliminate injured cells and maintain leukocyte homeostasis. Moreover, studies have suggested it regulates Fas-induced cytotoxicity and may have a non-apoptotic role in macrophage differentiation.

The SB125a monoclonal antibody detects a band at ~55kD corresponding to the full length form of caspase-8.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 1.0 mg/mL
Volume 0.1 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Western Blot – Quality tested
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795202
Gene ID
841 (Human)
Gene Symbol
CASP8 (Human)
Gene Aliases ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5
Uniprot ID
Q14790 (Human)
Uniprot Name
  • Total cell lysates from Jurkat cells were incubated with STS, resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-Human Caspase-8-UNLB (SB Cat. No. 17300-01). Proteins were visualized using Goat Anti-Rat IgM-HRP (SB Cat. No. 3020-05) secondary antibody and chemiluminescent detection.

Total cell lysates from Jurkat cells were incubated with STS, resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-Human Caspase-8-UNLB (SB Cat. No. 17300-01). Proteins were visualized using Goat Anti-Rat IgM-HRP (SB Cat. No. 3020-05) secondary antibody and chemiluminescent detection.
 
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