Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

Rat Anti-Mouse Ly-6A/E-LE/AF (D7)

Cat. No.:
1755-14
Low Endotoxin/Azide Free Anti-Mouse Ly-6A/E antibody for use in flow cytometry, immunohistochemistry, western blot, immunoprecipitation, and costimulation assays.
$380.00
Size Price (USD) Quantity
0.5 mg $380.00
More Information
Clone D7
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-LE/AF (KLH/G2a-1-1)
Specificity Mouse Ly-6A/E (Ly-6A.2 and Ly-6E.1)
Alternative Names Sca-1
Description Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. Studies with the monoclonal antibody D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.
Immunogen IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L
Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Handle under aseptic conditions
Applications Flow Cytometry – Quality tested 1,3-7
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunohistochemistry-Paraffin Sections – Reported in literature 2
Immunoprecipitation – Reported in literature 1,3
Western Blot – Reported in literature 1,3
Costimulation – Reported in literature 4

RRID Number AB_2795250
Gene ID 110454 (Mouse)
17069 (Mouse)
Gene ID Symbol Ly6a (Mouse)
Ly6e (Mouse)
Gene ID Aliases TAP; Sca1; Sca-1; Ly-6A.2; Ly-6A/E; Ly-6E.1 Ly67; RIG-E; Sca-2; TSA-1; Tsa1
UniProt ID P05533 (Mouse)
Q64253 (Mouse)
UniProt Name LY6A_MOUSE (Mouse)
LY6E_MOUSE (Mouse)

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
or call 800.722.2255
  1. 1. Ortega G, Korty PE, Shevach EM, Malek TR. Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity. J Immunol. 1986;137:3240-6. (Immunogen, FC, IP, WB)
  2. 2. van Bragt MP, Ciliberti N, Stanford WL, de Rooij DG, van Pelt AM. LY6A/E (SCA-1) expression in the mouse testis. Biol Reprod. 2005;73:634-8. (IHC-PS)
  3. 3. English A, Kosoy R, Pawlinski R, Bamezai A. A monoclonal antibody against the 66-kDa protein expressed in mouse spleen and thymus inhibits Ly-6A.2-dependent cell-cell adhesion. J Immunol. 2000;165:3763-71. (FC, IP, WB, IHC-FS)
  4. 4. Codias EK, Malek TR. Regulation of B lymphocyte responses to IL-4 and IFN-γ by activation through Ly-6A/E molecules. J Immunol. 1990;144:2197-204. (Costim, FC)
  5. 5. George J, Afek A, Abashidze A, Shmilovich H, Deutsch V, Kopolovich J, et al. Transfer of endothelial progenitor and bone marrow cells influences atherosclerotic plaque size and composition in apolipoprotein E knockout mice. Arterioscler Thromb Vasc Biol. 2005;25:2636-41. (FC)
  6. 6. Pevsner-Fischer M, Morad V, Cohen-Sfady M, Rousso-Noori L, Zanin-Zhorov A, Cohen S, et al. Toll-like receptors and their ligands control mesenchymal stem cell functions. Blood. 2007;109:1422-32. (FC)
  7. 7. Filip S, Mokrý J, Vávrová J, Šinkorová Z, Mičuda S, Šponer P, et al. The peripheral chimerism of bone marrow-derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice. J Cell Mol Med. 2014;18:832-43. (FC)
See All References