Rat Anti-Mouse Ly-6C-Alexa Fluor® 488

Cat. No.
1760-30  
Size
0.1 mg  
Price (USD)
$220.00 
Clone HK1.4
Isotype Rat (Lewis) IgG2cκ
Isotype Control Rat IgG2c-Alexa Fluor® 488
Immunogen L3 cloned CTL cells
Specificity Mouse Ly-6C
Description Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-8
Immunohistochemistry-Frozen Sections – Reported in literature 2
Activation – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795261
Gene ID
17067 (Mouse)
Gene Symbol
Ly6c1 (Mouse)
Gene Aliases AA682074; AA959465; Ly-6C; Ly-6C1; Ly6c
Uniprot ID
P0CW02 (Mouse)
Uniprot Name
  • BALB/c mouse bone marrow cells were stained with Rat Anti-Mouse Ly-6C-AF488 (SB Cat. No. 1760-30).

BALB/c mouse bone marrow cells were stained with Rat Anti-Mouse Ly-6C-AF488 (SB Cat. No. 1760-30).
 
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References
1. Harvan WL, Lancki DW, Moldwin RL, Dialynas DP, Fitch FW. Characterization of an anti-Ly-6 monoclonal antibody which defines and activates cytolytic T lymphocytes. J Immunol. 1988;140:1034-42. (Immunogen, FC, Activ)
2. Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, et al. Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion. J Immunol. 2008;180:3874-81. (FC, IHC-FS)
3. Chen CH, Grimbaldeston MA, Tsai M, Weissman IL, Galli SJ. Identification of mast cell progenitors in adult mice. Proc Natl Acad Sci USA. 2005;102:11408-13. (FC)
4. Chapatte L, Colombetti S, Cerottini J, Lévy F. Efficient induction of tumor antigen-specific CD8+ memory T cells by recombinant lentivectors. Cancer Res. 2006;66:1155-60. (FC)
5. Schröder J, Lüllmann-Rauch R, Himmerkus N, Pleines I, Nieswandt B, Orinska Z, et al. Deficiency of the tetraspanin CD63 associated with kidney pathology but normal lysosomal function. Mol Cell Biol. 2009;29:1083-94. (FC)
6. Sun Q, Jin H, Bond JS. Disruption of the meprin alpha and beta genes in mice alters homeostasis of monocytes and natural killer cells. Exp Hematol. 2009;37:346-56. (FC)
7. León B, del Hoyo GM, Parrillas V, Vargas HH, Sánchez-Mateos P, Longo N, et al. Dendritic cell differentiation potential of mouse monocytes: monocytes represent immediate precursors of CD8- and CD8+ splenic dendritic cells. Blood. 2004;103:2668-76. (FC)
8. Liu W, Xiao X, Demirci G, Madsen J, Li XC. Innate NK cells and macrophages recognize and reject allogeneic non-self in vivo via different mechanisms. J Immunol. 2012;188:2703-11. (FC)
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