Rat Anti-Mouse BiP-BIOT

Cat. No.
1775-08  
Size
0.5 mg   		Price (USD)
$305.00 
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Clone 76-E6
Isotype Rat (Fisher) IgG2aκ
Isotype Control Rat IgG2a-BIOT
Immunogen Mouse BiP:μ chain complexes
Specificity Mouse BiP
Alternalte Name(s) GRP78
Description The immunoglobulin heavy chain binding protein BiP is a member of the hsp70 family of heat shock proteins and is identical to the glucose regulated protein GRP78. While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp70 proteins have an essential physiological role in stress responses and as “molecular chaperones” which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding. With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (μ+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP. The monoclonal antibody 76-E6 recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Western Blot – Quality tested 2,3
Immunoprecipitation – Reported in literature 1
ELISA – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795269
Gene ID
14828 (Mouse)
Gene Symbol
Hspa5 (Mouse)
Gene Aliases Bip; Sez7; mBiP; Grp78; SEZ-7; Hsce70; baffled; AL022860; AU019543; D2Wsu17e; D2Wsu141e
Uniprot ID
P20029 (Mouse)
Uniprot Name
BIP_MOUSE 
  • Rat Anti-Mouse BiP-UNLB
    Total cell lysates from Ag8.653 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-Mouse BiP-UNLB (SB Cat. No. 1775-01). Proteins were visualized using Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05) secondary antibody and chemiluminescent detection.

Total cell lysates from Ag8.653 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-Mouse BiP-UNLB (SB Cat. No. 1775-01). Proteins were visualized using Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05) secondary antibody and chemiluminescent detection.
 
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References
1. Bole DG, Hendershot LM, Kearney JF. Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas. J Cell Biol. 1986;102:1558-66. (Immunogen, ELISA, IP)
2. Elkabetz Y, Argon Y, Bar-Nun S. Cysteines in CH1 underlie retention of unassembled Ig heavy chains. J Biol Chem. 2005;280:14402-12. (WB)
3. Elkabetz Y, Shapira I, Rabinovich E, Bar-Nun S. Distinct steps in dislocation of luminal endoplasmic reticulum-associated degradation substrates: roles of endoplamic reticulum-bound p97/Cdc48p and proteasome. J Biol Chem. 2004;279:3980-89. (WB)