Mouse Anti-Mouse Ly-49I-FITC
Cat. No.
1802-02
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Size
0.5 mg
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Price (USD)
$255.00
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Clone |
YLI-90
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Isotype |
Mouse (BALB/c) IgG1κ
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Isotype Control |
Mouse IgG1-FITC
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Immunogen |
CHO-K1 cells transfected with B6 allele of Ly49I gene
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Specificity |
Mouse Ly-49I
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Alternalte Name(s) |
Ly-49
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Description |
The monoclonal antibody YLI-90 reacts with the B6 allele of Ly49I, a member of the lectin-like homodimeric Ly49 family of cell surface receptors. Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The YLI-90 antibody stains ~45% of NK1.1+CD3- splenocytes from C57BL/6 mice.
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Format/Conjugate |
FITC (Fluorescein)
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Buffer Formulation |
Phosphate buffered saline containing 0.1% sodium azide
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Concentration |
0.5 mg/mL
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Volume |
1.0 mL
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Storage & Handling |
2-8°C; Avoid exposure to light
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - Flow Cytometry – Quality tested 1,2
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2795332
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Gene ID |
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Gene Symbol |
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Gene Aliases |
LY49I1; Ly49I
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-
C57BL/6 mouse splenocytes were stained with Mouse Anti-Mouse Ly-49I-BIOT (SB Cat. 1802-08) and Mouse Anti-Mouse NK1.1-PE (SB Cat. No. 1805-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
C57BL/6 mouse splenocytes were stained with Mouse Anti-Mouse Ly-49I-BIOT (SB Cat. 1802-08) and Mouse Anti-Mouse NK1.1-PE (SB Cat. No. 1805-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
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References
1. | Coles MC, McMahon CW, Takizawa H, Raulet DH. Memory CD8 T lymphocytes express inhibitory MHC-specific Ly49 receptors. Eur J Immunol. 2000;30:236-44. (Immunogen, FC) | 2. | Corral L, Takizawa H, Hanke T, Jamieson AM, Raulet DH. A new monoclonal antibody reactive with several Ly49 NK cell receptors mediates redirected lysis of target cells. Hybridoma. 1999;18:359-66. (FC) |
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