Rat Anti-Mouse NKG2-A/C/E-PE

Cat. No.
1804-09  
Size
0.1 mg   		Price (USD)
$180.00 
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Clone 20d5
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-PE
Immunogen CHO cells transfected with B6 allele of NKG2A gene
Specificity Mouse NKG2-A/C/E
Alternalte Name(s) NKG2A, NKG2C, NKG2E
Description The monoclonal antibody 20d5 reacts with an epitope common to the NKG2A, NKG2C and NKG2E isoforms of the NKG2 heterodimer, a member of the c-type lectin family of inhibitory receptors. Mouse NKG2 is a natural killer (NK) cell receptor for the non-classical MHC class I molecule Qa-1b. NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells. Two-color immunofluorescent staining with the 20d5 antibody reveals two distinct NKG2+ phenotypes within the NK1.1+CD3- spleen cell population: NKG2hi (50%) and NKG2low (50%). With the exception of DBA/2J mice, NKG2 is expressed on NK1.1+ cells from all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).
Format/Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-3
Blocking – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795343
Gene ID
16641 (Mouse)
16642 (Mouse)
58179 (Mouse)
Gene Symbol
Klrc1 (Mouse)
Klrc2 (Mouse)
Klrc3 (Mouse)
Gene Aliases NKG2A; NKG2B; CD159a; NKG2C; Klrc2; Nkg2e
  • Rat Anti-Mouse NKG2-A/C/E-PE
    C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse NKG2-A/C/E-PE (SB Cat. No. 1804-09) and Mouse Anti-Mouse NK1.1-FITC (SB Cat. No. 1805-02).

C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse NKG2-A/C/E-PE (SB Cat. No. 1804-09) and Mouse Anti-Mouse NK1.1-FITC (SB Cat. No. 1805-02).
 
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References
1. Vance RE, Jamieson AM, Raulet DH. Recognition of the class Ib molecule Qa-1b by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells. J Exp Med. 1999;190:1801-12. (Immunogen, FC, Block)
2. Fraser KP, Gays F, Robinson JH, van Beneden K, Leclercq G, Vance RE, et al. NK cells developing in vitro from fetal mouse progenitors express at least one member of the Ly49 family that is acquired in a time-dependent and stochastic manner independently of CD94 and NKG2. Eur J Immunol. 2002;32:686-78. (FC)
3. Lian RH, Maeda M, Lohwasser S, Delcommenne M, Nakano T, Vance RE, et al. Orderly and nonstochastic acquisition of CD94/NKG2 receptors by developing NK cells derived from embryonic stem cells in vitro. J Immunol. 2002;168:4980-7. (FC)