Mouse Anti-Mouse NK1.1-Alexa Fluor® 700

Cat. No.
1805-27  
Size
0.1 mg   		Price (USD)
$192.00 
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Clone PK136
Isotype Mouse (C3H x BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-Alexa Fluor® 700
Immunogen CE mouse spleen enriched for NK-1+ cells and bone marrow cells
Specificity Mouse NK1.1
Alternalte Name(s) Ly-55, CD161b/CD161c
Description NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL/6, NZB, and CE). NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.
Format/Conjugate AF700 (Alexa Fluor® 700)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,3-5,8,9
Immunohistochemistry –Reported in literature 8
Immunoprecipitation – Reported in literature 3,4
Depletion – Reported in literature 2,7
Complement Mediated Cell Depletion – Reported in literature 5
Blocking – Reported in literature 4
Activation – Reported in literature 6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795353
Gene ID
17059 (Mouse)
Gene Symbol
Klrb1c (Mouse)
Gene Aliases AI462337; CD161; Klrb1b; Ly-59; Ly55c; Ly59; NK-RP1; NK1.1; NKR-P1.9; NKRP1; NKRP140; Nk-1; Nk-1.2; Nk1; Nk1.2; Nkrp1b; Nkrp1c; ly-55c
Uniprot ID
P27814 (Mouse)
Uniprot Name
KLRBC_MOUSE 
  • Mouse Anti-Mouse NK1.1-PE
    C57BL/6 mouse splenocytes were stained with Mouse Anti-Mouse NK1.1-PE (SB Cat. No. 1805-09L) and Rat Anti-Mouse CD49b-FITC (SB Cat. No. 1806-02).

C57BL/6 mouse splenocytes were stained with Mouse Anti-Mouse NK1.1-PE (SB Cat. No. 1805-09L) and Rat Anti-Mouse CD49b-FITC (SB Cat. No. 1806-02).
 
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References
1. Koo GC, Peppard JR. Establishment of monoclonal anti-Nk-1.1 antibody. Hybridoma. 1984;3:301-3. (Immunogen, FC)
2. Koo GC, Dumont FJ, Tutt M, Hackett J Jr, Kumar V. The NK-1.1(-) mouse: a model to study differentiation of murine NK cells. J Immunol. 1986;137:3742-7. (Depletion)
3. Sentman CL, Hackett J Jr, Moore TA, Tutt MM, Bennett M, Kumar V. Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen. Hybridoma. 1989;8:605-14. (FC, IP)
4. Kung SK, Su R, Shannon J, Miller RG. The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells. J Immunol. 1999;162:5876-87. (FC, IP, Block)
5. Karlhofer FM, Yokoyama WM. Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways. J Immunol. 1991;146:3662-73. (FC, CMDC)
6. Reichlin A, Yokoyama WM. Natural killer cell proliferation induced by anti-NK1.1 and IL-2. Immunol Cell Biol. 1998;76:143-52. (Activ)
7. Sharma N, He Q, Sharma RP. Amelioration of fumonisin B1 hepatotoxicity in mice by depletion of T cells with anti-Thy-1.2. Toxicology. 2006;223:191-201. (Depletion)
8. Al-Falahi Y, Sand KL, Knudsen E, Damaj BB, Rolin J, Maghazachi AA. Splenic natural killer cell activity in two models of experimental neurodegenerative diseases. J Cell Mol Med. 2009;13:2693-703. (FC, IHC)
9. Freitas CS, Dalmau SR, Abdelhay E. Differential expression of notch signaling-related transcripts accompanies pro-thymocyte proliferation and phenotype transition induced by epidermal growth factor plus insulin in fetal thymus organ cultures. Mem Inst Oswaldo Cruz. 2004;99:381-88. (FC)
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