Rat Anti-Mouse CD24-LE/AF
Cat. No.
1815-14
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Size
0.5 mg
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Price (USD)
$300.00
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Clone |
30-F1
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Isotype |
Rat (LOU/Ws1/M) IgG2cκ
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Isotype Control |
Rat IgG2c-LE/AF
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Immunogen |
Mouse thymus or spleen
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Specificity |
Mouse CD24
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Alternalte Name(s) |
Heat stable antigen, HSA
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Description |
CD24 is expressed in B cells until their terminal differentiation into plasma cells. It has been used widely as a marker to fractionate different B-lineage differentiation stages. CD24 has been found on a variety of other hematopoietic and neural cell types and is now classified as a costimulatory molecule. The CD24 molecule, also known as HSA, is composed mostly of carbohydrate with a protein core of only 31-35 amino acids in the human and 27 amino acids in the mouse. CD24 is now included in the large group of phosphatidylinositol-anchored membrane proteins. Studies with purified CD24 applied to latex beads have demonstrated a self-binding property suggesting that the molecule is capable of promoting homotypic aggregation, binding, and cell adhesion. This is supported by earlier observations of the blockade of B cell lymphoblast aggregation with an anti-CD24 monoclonal antibody. Recent studies of murine CD24 have assigned a role for CD24 as a ligand for P-selectin, a calcium-dependent lectin.
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Format/Conjugate |
LE/AF (Low Endotoxin/Azide Free)
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Buffer Formulation |
Phosphate buffered saline, pH 7.4
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Concentration |
0.5 mg/mL
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Volume |
1.0 mL
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Storage & Handling |
-20°C
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - Flow Cytometry – Quality tested 1-4
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2795392
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Gene ID |
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Gene Symbol |
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Gene Aliases |
HSA; Cd24; Ly-52; nectadrin
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Uniprot ID |
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Uniprot Name |
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-
C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD24-APC (SB Cat. No. 1815-11) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD24-APC (SB Cat. No. 1815-11) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
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References
1. | Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. (Immunogen, FC) | 2. | Palmer VL, Kassmeier MD, Willcockson J, Akhter MP, Cullen DM, Swanson PC. N-acetylcysteine increases the frequency of bone marrow pro-B/pre-B cells, but does not reverse cigarette smoking-induced loss of this subset. PLoS One. 2011;6:e24804. (FC) | 3. | Hassaballa AE, Palmer VL, Anderson DK, Kassmeier MD, Nganga VK, Parks KW, et al. Accumulation of B1-like B cells in transgenic mice over-expressing catalytically inactive RAG1 in the periphery. Immunology. 2011;134:469-86. (FC) | 4. | Kassmeier MD, Mondal K, Palmer VL, Ravel P, Kumar S, Perry GA, et al. VprBP binds full-length RAG1 and is required for B-cell development and V(D)J recombination fidelity. EMBO J. 2012;31:945-58. (FC) |
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