Mouse Anti-Mouse I-Ek-FITC

Cat. No.
1898-02  
Size
0.5 mg  
Price (USD)
$275.00 
Clone 17-3-3
Isotype Mouse (C3H.SW) IgG2aκ
Isotype Control Mouse IgG2a-FITC
Immunogen C3H mouse skin graft and splenocytes
Specificity Mouse I-Ek
Alternalte Name(s) MHC Class II
Description The monoclonal antibody 17-3-3 reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype. Reactivity with H-2d and H-2p haplotypes has not been observed. Cells from mice of the H-2b, H-2f, H-2g, H-2q, and H-2s haplotypes do not express I-E antigen.
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 3
Immunohistochemistry-Frozen Sections – Reported in literature 2
Complement Mediated Cell Depletion – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795459
Gene ID
14969 (Mouse)
Gene Symbol
H2-Eb1 (Mouse)
Gene Aliases Eb; Ia4; H2Eb; Ia-4; H-2Eb
Uniprot ID
P18469 (Mouse)
Uniprot Name
  • AKR mouse splenocytes were stained with Mouse Anti-Mouse I-Ek-FITC (SB Cat. No. 1898-02) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).

AKR mouse splenocytes were stained with Mouse Anti-Mouse I-Ek-FITC (SB Cat. No. 1898-02) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
 
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References
1. Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980;124:533-40. (Immunogen, CMDC)
2. Colson YL, Lange J, Fowler K, Ildstad ST. Mechanism for cotolerance in nonlethally conditioned mixed chimeras: negative selection of the Vbeta T-cell receptor repertoire by both host and donor bone marrow-derived cells. Blood. 1996;88:4601-10. (IHC-FS)
3. Anderson GD, David CS. In vivo expression and function of hybrid Ia dimers (EαAβ) in recombinant and transgenic mice. J Exp Med. 1989;170:1003-8. (FC)