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Mouse Anti-Mouse I-Ek-UNLB (17-3-3)

Cat. No.:
Purified Anti-Mouse I-Ek antibody for use in flow cytometry, immunohistochemistry, and CMCD assays.
Size Price (USD) Quantity
0.5 mg $234.00
More Information
Clone 17-3-3
Isotype Mouse (C3H.SW) IgG2aκ
Isotype Control Mouse IgG2a-UNLB (HOPC-1)
Specificity Mouse I-Ek
Alternative Names MHC Class II
Description The monoclonal antibody 17-3-3 reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype. Reactivity with H-2d and H-2p haplotypes has not been observed. Cells from mice of the H-2b, H-2f, H-2g, H-2q, and H-2s haplotypes do not express I-E antigen.
Immunogen C3H mouse skin graft and splenocytes
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 3
Immunohistochemistry-Frozen Sections – Reported in literature 2
Complement Mediated Cell Depletion – Reported in literature 1

RRID Number AB_2795458
Gene ID 14969 (Mouse)
Gene ID Symbol H2-Eb1 (Mouse)
Gene ID Aliases Eb; Ia4; H2Eb; Ia-4; H-2Eb
UniProt ID P18469 (Mouse
UniProt Name HB2J_MOUSE (Mouse)

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  1. 1. Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980;124:533-40. (Immunogen, CMDC)
  2. 2. Colson YL, Lange J, Fowler K, Ildstad ST. Mechanism for cotolerance in nonlethally conditioned mixed chimeras: negative selection of the Vbeta T-cell receptor repertoire by both host and donor bone marrow-derived cells. Blood. 1996;88:4601-10. (IHC-FS)
  3. 3. Anderson GD, David CS. In vivo expression and function of hybrid Ia dimers (EαAβ) in recombinant and transgenic mice. J Exp Med. 1989;170:1003-8. (FC)