Mouse Anti-Mouse I-Ad-UNLB

Cat. No.
1901-01  
Size
0.5 mg  
Price (USD)
$212.00 
Clone 34-5-3
Isotype Mouse (C3H) IgG2aκ
Isotype Control Mouse IgG2a-UNLB
Immunogen (C57BL/6 x DBA/2)F1 mouse splenocytes
Specificity Mouse I-Ad
Alternalte Name(s) MHC Class II, I-bd, I-Aβ, H2-I/Adβ
Description The monoclonal antibody 34-5-3 reacts with the β chain of the I-Ad MHC class II alloantigen. It cross-reacts with I-Ab and with cells from mice of the H-2p and H-2q halotypes.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 5
Immunoprecipitation – Reported in literature 2
Activation – Reported in literature 4
Blocking – Reported in literature 3
Cytoxicity – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795478
Gene ID
14961 (Mouse)
Gene Symbol
H2-Ab1 (Mouse)
Gene Aliases IAb; Ia2; Ia-2; Abeta; H-2Ab; H2-Ab; Rmcs1; I-Abeta; AI845868
Uniprot ID
P01921 (Mouse)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Mouse Anti-Mouse I-Ad-UNLB (SB Cat. No. 1901-01) and Rat Anti-Mouse CD3ε-FITC (SB Cat. No. 1535-02) followed by Goat Anti-Mouse IgG2a, Human ads-PE (SB Cat. No. 1080-09).
  • BALB/c mouse splenocytes were stained with Mouse Anti-Mouse I-Ad-UNLB (SB Cat. No. 1901-01) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09) followed by Goat Anti-Mouse IgG2a, Human ads-FITC (SB Cat. No. 1080-02).

BALB/c mouse splenocytes were stained with Mouse Anti-Mouse I-Ad-UNLB (SB Cat. No. 1901-01) and Rat Anti-Mouse CD3ε-FITC (SB Cat. No. 1535-02) followed by Goat Anti-Mouse IgG2a, Human ads-PE (SB Cat. No. 1080-09).
 

BALB/c mouse splenocytes were stained with Mouse Anti-Mouse I-Ad-UNLB (SB Cat. No. 1901-01) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09) followed by Goat Anti-Mouse IgG2a, Human ads-FITC (SB Cat. No. 1080-02).
 
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References
1. Ozato K, Mayer NM, Sachs DH. Monoclonal antibodies to mouse major histocompatibility complex antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. Transplantation. 1982;34:113-9. (Immunogen, Cyto)
2. Bobé P, Benihoud K, Kiger N. Allogenic MHC class II determinant(s) in MRL-Ipr autoimmune disease-prone mice. Unusual expression of an L1 transposable element creates molecular mimicry. J Immunol. 1993;151:2813-9. (IP)
3. Mozes E, Dayan M, Zisman E, Brocke S, Licht A, Pecht I. Direct binding of a myasthenia gravis related epitope to MHC class II molecules on living murine antigen-presenting cells. EMBO J. 1989;8:4049-52. (Block)
4. Bishop GA, Frelinger JA. Haplotype-specific differences in signaling by transfected class II molecules to a Ly-1+ B-cell clone. Proc Natl Acad Sci USA. 1989;86:5933-7. (Activ)
5. Grusby MJ, Johnson RS, Papaioannou VE, Glimcher LH. Deletion of CD4+ T cells in major histocompatibility complex class II-deficient mice. Science. 1991;253:1417-20. (FC)