Mouse Anti-Mouse H-2Kd/H-2Dd-FITC
Cat. No.
1911-02
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Size
0.5 mg
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Price (USD)
$245.00
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Clone |
34-1-2S
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Isotype |
Mouse (C3H) IgG2aκ
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Isotype Control |
Mouse IgG2a-FITC
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Immunogen |
BDF1 mouse splenocytes
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Specificity |
Mouse H-2Kd/H-2Dd
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Alternalte Name(s) |
MHC Class I
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Description |
The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic α chains noncovalently associated with the invariant β2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 34-1-2S binds to a common determinant in the α3 domains of H-2Kd and H-2Dd in the presence or absence of β2 microglobulin. It cross reacts with the α3 domain of H-2Kb.
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Format/Conjugate |
FITC (Fluorescein)
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Buffer Formulation |
Phosphate buffered saline containing 0.1% sodium azide
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Concentration |
0.5 mg/mL
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Volume |
1.0 mL
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Storage & Handling |
2-8°C; Avoid exposure to light
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - Flow Cytometry – Quality tested 3,4 Immunocytochemistry – Reported in literature 3,5 Electron Microscopy – Reported in literature 4 Immunoprecipitation – Reported in literature 2 Complement Mediated Cell Depletion – Reported in literature 3 Blocking – Reported in literature 6 Adhesion – Reported in literature 6
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2795497
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Gene ID |
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Gene Symbol |
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Gene Aliases |
K-f; H-2K; H2-K; H2-D1; H-2K(d); H-2D; H2-D; H2-K1
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Uniprot ID |
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Uniprot Name |
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BALB/c mouse splenocytes were stained with Mouse Anti-Mouse H-2Kd/H-2Dd-PE (SB Cat. No. 1911-09).
BALB/c mouse splenocytes were stained with Mouse Anti-Mouse H-2Kd/H-2Dd-PE (SB Cat. No. 1911-09).
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References
1. | Ozato K, Mayer NM, Sachs DH. Monoclonal antibodies to mouse major histocompatibility complex antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. Transplantation. 1982;34:113-9. (Immunogen) | 2. | Burgert H, Maryanski JL, Kvist S. "E3/19K" protein of adenovirus type 2 inhibits lysis of cytolytic T lymphocytes by blocking cell-surface expression of histocompatibility class I antigens. Proc Natl Acad Sci USA. 1987;84:1356-60. (IP) | 3. | Lenz A, Heufler C, Rammensee H, Glassl H, Koch F, Romani N, et al. Murine epidermal Langerhans cells express significant amounts of class I major histocompatibility complex antigens. Proc Natl Acad Sci USA. 1989;86:7527-31. (FC, ICC, CMDC) | 4. | Pereira RA, Simmons A. Cell surface expression of H2 antigens on primary sensory neurons in response to acute but not latent herpes simplex virus infection in vivo. J Virol. 1999;73:6484-9. (FC, EM) | 5. | Abendroth A, Simmons A, Efstathiou S, Pereira RA. Infection with an H2 recombinant herpes simplex virus vector results in expression of MHC class I antigens on the surfaces of human neuroblastoma cells in vitro and mouse sensory neurons in vivo. J Gen Virol. 2000;81:2375-83. (ICC) | 6. | Brennen J, Mager D, Jefferies W, Takei F. Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties. J Exp Med. 1994;180:2287-95. (Block, Adhesion) |
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