Mouse Anti-Mouse H-2Kd/H-2Dd-FITC

Cat. No.
1911-02  
Size
0.5 mg  
Price (USD)
$245.00 
Clone 34-1-2S
Isotype Mouse (C3H) IgG2aκ
Isotype Control Mouse IgG2a-FITC
Immunogen BDF1 mouse splenocytes
Specificity Mouse H-2Kd/H-2Dd
Alternalte Name(s) MHC Class I
Description The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic α chains noncovalently associated with the invariant β2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 34-1-2S binds to a common determinant in the α3 domains of H-2Kd and H-2Dd in the presence or absence of β2 microglobulin. It cross reacts with the α3 domain of H-2Kb.
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 3,4
Immunocytochemistry – Reported in literature 3,5
Electron Microscopy – Reported in literature 4
Immunoprecipitation – Reported in literature 2
Complement Mediated Cell Depletion – Reported in literature 3
Blocking – Reported in literature 6
Adhesion – Reported in literature 6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795497
Gene ID
14972 (Mouse)
14964 (Mouse)
Gene Symbol
H2-K1 (Mouse)
H2-D1 (Mouse)
Gene Aliases K-f; H-2K; H2-K; H2-D1; H-2K(d); H-2D; H2-D; H2-K1
Uniprot ID
P01902 (Mouse)
P01900 (Mouse)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Mouse Anti-Mouse H-2Kd/H-2Dd-PE (SB Cat. No. 1911-09).

BALB/c mouse splenocytes were stained with Mouse Anti-Mouse H-2Kd/H-2Dd-PE (SB Cat. No. 1911-09).
 
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References
1. Ozato K, Mayer NM, Sachs DH. Monoclonal antibodies to mouse major histocompatibility complex antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. Transplantation. 1982;34:113-9. (Immunogen)
2. Burgert H, Maryanski JL, Kvist S. "E3/19K" protein of adenovirus type 2 inhibits lysis of cytolytic T lymphocytes by blocking cell-surface expression of histocompatibility class I antigens. Proc Natl Acad Sci USA. 1987;84:1356-60. (IP)
3. Lenz A, Heufler C, Rammensee H, Glassl H, Koch F, Romani N, et al. Murine epidermal Langerhans cells express significant amounts of class I major histocompatibility complex antigens. Proc Natl Acad Sci USA. 1989;86:7527-31. (FC, ICC, CMDC)
4. Pereira RA, Simmons A. Cell surface expression of H2 antigens on primary sensory neurons in response to acute but not latent herpes simplex virus infection in vivo. J Virol. 1999;73:6484-9. (FC, EM)
5. Abendroth A, Simmons A, Efstathiou S, Pereira RA. Infection with an H2 recombinant herpes simplex virus vector results in expression of MHC class I antigens on the surfaces of human neuroblastoma cells in vitro and mouse sensory neurons in vivo. J Gen Virol. 2000;81:2375-83. (ICC)
6. Brennen J, Mager D, Jefferies W, Takei F. Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties. J Exp Med. 1994;180:2287-95. (Block, Adhesion)