Goat Anti-Human Ig Fc-UNLB

Cat. No.
2047-01  
Size
0.5 mg  
Price (USD)
$70.00 
Specificity Reacts with the Fc region of human IgG, IgM, and IgA
Source Pooled antisera from goats hyperimmunized with human IgG, IgM, and IgA
Cross Adsorption Human IgG Fab; may react with immunoglobulins from other species and the Fc region of other human immunoglobulins
Purification Affinity chromatography on pooled human immunoglobulins covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795681
  • ELISA plate was coated with purified human IgG, IgM, IgA, IgG Fc, and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Human Ig Fc-UNLB (SB Cat. No. 2047-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).

ELISA plate was coated with purified human IgG, IgM, IgA, IgG Fc, and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Human Ig Fc-UNLB (SB Cat. No. 2047-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
 
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References
1. Terato K, Do CT, Cutler D, Waritani T, Shionoya H. Preventing intense false positive and negative reactions attributed to the principle of ELISA to re-investigate antibody studies in autoimmune diseases. J Immunol Methods. 2014;407:15-25. (ELISA)
2. Zhao Q, Ahmed M, Guo H, Cheung IY, Cheung NV. Alteration of electrostatic surface potential enhances affinity and tumor killing properties of anti-ganglioside GD2 monoclonal antibody hu3F8. J Biol Chem. 2015;290:13017-27. (ELISA)