Mouse Anti-His-Tag-BIOT

Cat. No.
4603-08  
Size
0.5 mg  
Price (USD)
$345.00 
Clone SB194b
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-BIOT
Immunogen Synthetic 6X His-tag peptides
Specificity His-tag
Reacts with N-terminal, C-terminal, and internal repeating histidine residues of 6 or more
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1
Western blot 1
Immunoprecipitation 1
Immunocytochemistry 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • CHO-K1 cells transfected with GFP - N-terminal 6X His-tag were stained with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
  • Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
  • ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).
  • CHO-K1 cell lysates transfected with GFP - 6X His-tag were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
  • ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA and BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
  • Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
  • ELISA plate was coated with C-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).
  • TEV protease / maltose binding protein (MBP) fusion protein with an internal 6X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) secondary antibody and chemiluminescent detection.

CHO-K1 cells transfected with GFP - N-terminal 6X His-tag were stained with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
 

Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
 

ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 

CHO-K1 cell lysates transfected with GFP - 6X His-tag were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
 

ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA and BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 

Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
 

ELISA plate was coated with C-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 

TEV protease / maltose binding protein (MBP) fusion protein with an internal 6X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) secondary antibody and chemiluminescent detection.
 
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References
1. SouthernBiotech published data