Cat. No.
4603-08

Size
0.5 mg

Price (USD)
$345.00

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Clone	SB194b
Isotype	Mouse (BALB/c) IgG₁κ
Isotype Control	Mouse IgG₁-BIOT
Immunogen	Synthetic 6X His-tag peptides
Specificity	His-tag Reacts with N-terminal, C-terminal, and internal repeating histidine residues of 6 or more
Format/Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA – Quality tested ¹ FLISA – Quality tested ¹ Immunoprecipitation – Quality tested ¹ Western blot ¹ Purification ¹ Flow Cytometry ¹ Immunocytochemistry ¹
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2819137

CHO-K1 cells transfected with GFP - N-terminal 6X His-tag were stained with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).
CHO-K1 cell lysates transfected with GFP - 6X His-tag were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA and BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
ELISA plate was coated with C-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).
TEV protease / maltose binding protein (MBP) fusion protein with an internal 6X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) secondary antibody and chemiluminescent detection.

CHO-K1 cells transfected with GFP - N-terminal 6X His-tag were stained with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.

Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.

ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).

CHO-K1 cell lysates transfected with GFP - 6X His-tag were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.

ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA and BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.

ELISA plate was coated with C-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).

TEV protease / maltose binding protein (MBP) fusion protein with an internal 6X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) secondary antibody and chemiluminescent detection.