Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.