Mouse Anti-His-Tag-SEPH

Cat. No.
4603-25  
Size
25 tests  
Price (USD)
$295.00 
Clone SB194b
Isotype Mouse (BALB/c) IgG1κ
Immunogen Synthetic 6X His-tag peptides
Specificity His-tag
Reacts with N-terminal, C-terminal, and internal repeating histidine residues of 6 or more
Format/Conjugate SEPH (Agarose)
Buffer Formulation Borate buffered saline, pH 8.2
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1
FLISA – Quality tested 1
Immunoprecipitation – Quality tested 1
Western blot 1
Purification 1
Flow Cytometry 1
Immunocytochemistry 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • E. coli expressed His-tagged fusion protein was purified from equivalent amounts of the same sample using Mouse Anti-His-Tag-SEPH (SB Cat. No. 4603-25; left) and Ni-NTA resin (right). Eluted fractions were concentrated and analyzed by SDS-PAGE.
  • His-tagged recombinant protein was immunoprecipitated from E. coli cell lysate with Mouse Anti-His-Tag-SEPH (SB Cat. No. 4603-25; Lane 2) and BSA linked to agarose beads (Lane 1). Lysate was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-HRP (SB Cat. No. 4603-05). Protein was visualized using chemiluminescent detection.

E. coli expressed His-tagged fusion protein was purified from equivalent amounts of the same sample using Mouse Anti-His-Tag-SEPH (SB Cat. No. 4603-25; left) and Ni-NTA resin (right). Eluted fractions were concentrated and analyzed by SDS-PAGE.
 

His-tagged recombinant protein was immunoprecipitated from E. coli cell lysate with Mouse Anti-His-Tag-SEPH (SB Cat. No. 4603-25; Lane 2) and BSA linked to agarose beads (Lane 1). Lysate was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-HRP (SB Cat. No. 4603-05). Protein was visualized using chemiluminescent detection.
 
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References
1. SouthernBiotech published data
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