Mouse Anti-His-Tag-Alexa Fluor® 488

Cat. No.
4603-30  
Size
0.1 mg  
Price (USD)
$345.00 
Clone SB194b
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-Alexa Fluor® 488
Immunogen Synthetic 6X His-tag peptides
Specificity His-tag
Reacts with N-terminal, C-terminal, and internal repeating histidine residues of 6 or more
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1
FLISA – Quality tested 1
Immunoprecipitation – Quality tested 1
Western blot 1
Purification 1
Flow Cytometry 1
Immunocytochemistry 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Human peripheral blood lymphocytes were stained with purified / unlabeled His-tagged recombinant Anti-Human IgM followed by Mouse Anti-His-Tag-AF488 (SB Cat. No. 4603-30).
  • FLISA plate was coated with N-terminal 6X His-tag conjugated to BSA and BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-AF488 (SB Cat. No. 4603-30).

Human peripheral blood lymphocytes were stained with purified / unlabeled His-tagged recombinant Anti-Human IgM followed by Mouse Anti-His-Tag-AF488 (SB Cat. No. 4603-30).
 

FLISA plate was coated with N-terminal 6X His-tag conjugated to BSA and BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-AF488 (SB Cat. No. 4603-30).
 
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References
1. SouthernBiotech published data
Alexa Fluor® is a registered trademark of Life Technologies Corporation