Rabbit Anti-Hamster IgG(H+L), Mouse/Rat SP ads-UNLB

Cat. No.
6215-01  
Size
0.5 mg  
Price (USD)
$90.00 
Specificity Reacts with the heavy and light chains of Syrian and Armenian hamster IgG
Source Pooled antisera from rabbits hyperimmunized with hamster IgG
Cross Adsorption Mouse and rat serum proteins (SP); may react with immunoglobulins from other species and the light chains of other hamster immunoglobulins
Purification Affinity chromatography on hamster IgG covalently linked to agarose
Isotype Rabbit IgG
Isotype Control Rabbit IgG-UNLB
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 1
Immunohistochemistry-Frozen Sections 2
Western Blot 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796275
  • ELISA plate was coated with purified Syrian hamster IgG, Armenian hamster IgG, mouse serum, and rat serum. Immunoglobulins and sera were detected with Rabbit Anti-Hamster IgG(H+L), Mouse/Rat SP ads-UNLB (SB Cat. No. 6215-01) followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05).

ELISA plate was coated with purified Syrian hamster IgG, Armenian hamster IgG, mouse serum, and rat serum. Immunoglobulins and sera were detected with Rabbit Anti-Hamster IgG(H+L), Mouse/Rat SP ads-UNLB (SB Cat. No. 6215-01) followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05).
 
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References
1. Nanki T, Urasaki Y, Imai T, Nishimura M, Muramoto K, Kubota T, et al. Inhibition of fractalkine ameliorates murine collagen-induced arthritis. J Immunol. 2004;173:7010-6. (FC)
2. Sajic D, Patrick AJ, Rosenthal KL. Mucosal delivery of CpG oligodeoxynucleotides expands functional dendritic cells and macrophages in the vagina. Immunology. 2005;114:213-24. (IHC-FS)
3. Priest JW, Moss DM, Won K, Todd CW, Henderson L, Jones CC, et al. Multiplex assay detection of immunoglobulin G antibodies that recognize Babesia microti antigens. Clin Vaccine Immunol. 2012;19:1539-48. (WB)