Donkey Anti-Rabbit IgG(H+L), Multi-Species SP ads-AP

Cat. No.
6442-04  
Size
1.0 mL  
Price (USD)
$150.00 
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Pooled antisera from donkeys hyperimmunized with rabbit IgG
Cross Adsorption Human, mouse, rat, bovine, horse, hamster, goat, sheep, chicken, and guinea pig serum proteins (SP); may react with immunoglobulins from other species and the light chains of other rabbit immunoglobulins
Purification Affinity chromatography on rabbit IgG covalently linked to agarose
Isotype Donkey IgG
Format/Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing 0.1% sodium azide, pH 8.0
Volume 1.0 mL mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Paraffin Sections 1
Immunocytochemistry 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796380
  • Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) and Rabbit IgG-UNLB (SB Cat. No. 0111-01; left) followed by Donkey F(ab’)2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF555 (SB Cat. No. 6442-32) and DAPI.

Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) and Rabbit IgG-UNLB (SB Cat. No. 0111-01; left) followed by Donkey F(ab’)2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF555 (SB Cat. No. 6442-32) and DAPI.
 
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References
1. SouthernBiotech published data (IHC-PS, ICC)