Mouse Anti-Human CD45-BIOT

Cat. No.
9624-08  
Size
100 tests  
Price (USD)
$158.00 
Clone 2D1
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-BIOT
Immunogen Human peripheral blood mononuclear cells
Specificity Human CD45
Alternalte Name(s) LCA, T200, Ly5
Description CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 6
Immunohistochemistry-Frozen Sections – Reported in literature 1-3
Immunohistochemistry-Paraffin Sections – Reported in literature 4
IP-Non-Reducing – Reported in literature 5,6
Western Blot-Non-Reducing – Reported in literature 5
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2797015
  • Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45-APC (SB Cat. No. 9624-11).

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45-APC (SB Cat. No. 9624-11).
 
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References
1. Gillitzer R, Berger R, Moll H. A reliable method for simultaneous demonstration of two antigens using a novel combination of immunogold-silver staining and immunoenzymatic labeling. J Histochem Cytochem. 1990;38:307-13. (IHC-FS)
2. Nickoloff BJ, Kunkel SL, Burdick M, Strieter RM. Severe combined immunodeficiency mouse and human psoriatic skin chimeras. Validation of a new animal model. Am J Pathol. 1995;146:580-8. (IHC-FS)
3. Ménétrier-Caux C, Bain C, Favrot MC, Duc A, Blay JY. Renal cell carcinoma induces interleukin 10 and prostaglandin E2 production by monocytes. Br J Cancer. 1999;79:119-30. (IHC-FS)
4. Charbord P, Tavian M, Humeau L, Péault B. Early ontogeny of the human marrow from long bones: an immunohistochemical study of hematopoiesis and its microenvironment. Blood. 1996;87:4109-19. (IHC-PS)
5. Poggi A, Pardi R, Pella N, Morelli L, Sivori S, Vitale M, et al. CD45-mediated regulation of LFA1 function in human natural killer cells. Anti-CD45 monoclonal antibodies inhibit the calcium mobilization induced via LFA1 molecules. Eur J Immunol. 1993;23:2454-63. (IP, WB)
6. Lazarovits AI, Osman N, Le Feuvre CE, Ley SC, Crumpton MJ. CD7 is associated with CD3 and CD45 on human T cells. J Immunol. 1994;153:3956-66. (IP, FC)