Rat Anti-Mouse CD3ε-APC

Cat. No.
0.1 mg  
Price (USD)
Clone C363.29B
Isotype Rat (Lewis) IgG2bκ
Isotype Control Rat IgG2b-APC
Immunogen IL-4 producing Th2 cell lines including D10
Specificity Mouse CD3ε
Alternalte Name(s) CD3 epsilon
Description CD3ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25 kDa ε chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.2B9 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The monoclonal antibody is cytolytic, easily used for cell surface staining, and a good immunoprecipitating antibody.
Format/Conjugate APC (Allophycocyanin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,7-12
Immunohistochemistry-Frozen Sections – Reported in literature 2-4
Immunohistochemistry-Paraffin Sections – Reported in literature 5,6
Immunoprecipitation – Reported in literature 1
Depletion – Reported in literature 1
Activation – Reported in literature 1,4
Complement Mediated Cell Depletion – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794824
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD3ε-APC (SB Cat. No. 1535-11) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD3ε-APC (SB Cat. No. 1535-11) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
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1. Portoles P, Rojo J, Golby A, Bonneville M, Gromkowski S, Greenbaum K, et al. Monoclonal antibodies to murine CD3ε define distinct epitopes one of which may interact with CD4 during T cell activation. J Immunol. 1989;142:4169-75. (Immunogen, FC, IP, Depletion, Activ, CMDC)
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