Mouse Anti-Rat IgG1-Alexa Fluor® 647

Cat. No.
3061-31  
Size
0.1 mg  
Price (USD)
$250.00 
Clone G11C5
Isotype Mouse (BALB/c) IgG2bκ
Immunogen Rat IgG1 hybridoma
Specificity Rat IgG1
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested
ELISA – Quality tested
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 1,2
ELISpot – Reported in literature 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795868
  • FLISA plate was coated with purified rat IgG1, IgG2a, IgG2b, IgG2c, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG1-AF647 (SB Cat. No. 3061-31).

FLISA plate was coated with purified rat IgG1, IgG2a, IgG2b, IgG2c, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG1-AF647 (SB Cat. No. 3061-31).
 
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References
1. Birjandi SZ, Ippolito JA, Ramadorai AK, Witte PL. Alterations in marginal zone macrophages and marginal zone B cells in old mice. J Immunol. 2011;186:3441-51. (IHC-FS)
2. Bahamon E. Evidence for renewal and reconstitution of marginal zone macrophages in young and aged mice [thesis]. Chicago (IL): Loyola University Chicago; 2013. (IHC-FS)
3. Sharkar MT, Jou M, Hossain MB, Lönnerdal B, Stephensen CB, Raqib R. Prenatal zinc supplementation of zinc-adequate rats adversely affects immunity in offspring. J Nutr. 2011;141:1559-64. (ELISPOT)
Alexa Fluor® is a registered trademark of Life Technologies Corporation